These reports summarize bat calls collected along transects at Clarks River National Wildlife Refuge for the CY 2016. Calls were classified using Bat Call ID software and restricted to calls with 5 or more pulses. This annual summary report contains summary information on transect surveys, species detections, maps of species locations. Summary tables include all classified species observations including those that did not have an associated spatial reference. The report summarizes acoustical bat data from a survey route run at Clarks River National Wildlife Refuge in 2016.