@prefix dcat: <http://www.w3.org/ns/dcat#> .
@prefix dct: <http://purl.org/dc/terms/> .
@prefix foaf: <http://xmlns.com/foaf/0.1/> .
@prefix gsp: <http://www.opengis.net/ont/geosparql#> .
@prefix locn: <http://www.w3.org/ns/locn#> .
@prefix vcard: <http://www.w3.org/2006/vcard/ns#> .
@prefix xsd: <http://www.w3.org/2001/XMLSchema#> .

<https://data.amerigeoss.org/dataset/088f58ab-0516-40f6-8004-9d40a03096cb> a dcat:Dataset ;
    dct:description """<p>This dataset contains files reconstructing single-cell data presented in &#039;Reference transcriptomics of porcine peripheral immune cells created through bulk and single-cell RNA sequencing&#039; by Herrera-Uribe & Wiarda et al. 2021. Samples of peripheral blood mononuclear cells (PBMCs) were collected from seven pigs and processed for single-cell RNA sequencing (scRNA-seq) in order to provide a reference annotation of porcine immune cell transcriptomics at enhanced, single-cell resolution. Analysis of single-cell data allowed identification of 36 cell clusters that were further classified into 13 cell types, including monocytes, dendritic cells, B cells, antibody-secreting cells, numerous populations of T cells, NK cells, and erythrocytes. Files may be used to reconstruct the data as presented in the manuscript, allowing for individual query by other users. Scripts for original data analysis are available at <a href="https://github.com/USDA-FSEPRU/PorcinePBMCs_bulkRNAseq_scRNAseq">https://github.com/USDA-FSEPRU/PorcinePBMCs_bulkRNAseq_scRNAseq</a>. Raw data are available at <a href="https://www.ebi.ac.uk/ena/browser/view/PRJEB43826">https://www.ebi.ac.uk/ena/browser/view/PRJEB43826</a>.</p>
<p>Funding for this dataset was also provided by NRSP8: National Animal Genome Research Program (<a href="https://www.nimss.org/projects/view/mrp/outline/18464">https://www.nimss.org/projects/view/mrp/outline/18464</a>).</p>
""" ;
    dct:identifier "673fd36c-e9a0-4522-b6f1-cb68c54df85b" ;
    dct:issued "2025-11-19T21:25:47.930609"^^xsd:dateTime ;
    dct:modified "2022-01-05"^^xsd:date ;
    dct:publisher <https://data.amerigeoss.org/organization/727dbdd5-3f98-4ac0-9d28-5e344558139b> ;
    dct:spatial [ a dct:Location ;
            locn:geometry "POLYGON ((-93.7354 41.9374, -93.7354 42.1169, -93.4827 42.1169, -93.4827 41.9374))"^^gsp:wktLiteral ] ;
    dct:title "Data from: Reference transcriptomics of porcine peripheral immune cells created through bulk and single-cell RNA sequencing" ;
    dcat:contactPoint [ a vcard:Organization ;
            vcard:fn "Loving, Crystal" ;
            vcard:hasEmail <mailto:crystal.loving@usda.gov> ] ;
    dcat:distribution <https://data.amerigeoss.org/dataset/088f58ab-0516-40f6-8004-9d40a03096cb/resource/0512a6ad-0c61-430a-8ad2-9b79eafd625b>,
        <https://data.amerigeoss.org/dataset/088f58ab-0516-40f6-8004-9d40a03096cb/resource/29ba57ad-b7e2-4825-936f-46156163471d>,
        <https://data.amerigeoss.org/dataset/088f58ab-0516-40f6-8004-9d40a03096cb/resource/3ecd5b03-7aac-4669-b2e5-107aa2318368>,
        <https://data.amerigeoss.org/dataset/088f58ab-0516-40f6-8004-9d40a03096cb/resource/54cd0a40-4d60-417f-a546-eb408c3a642a>,
        <https://data.amerigeoss.org/dataset/088f58ab-0516-40f6-8004-9d40a03096cb/resource/66ea6bbe-8cf9-471c-8449-2888109a951c>,
        <https://data.amerigeoss.org/dataset/088f58ab-0516-40f6-8004-9d40a03096cb/resource/6a2a8452-5eb5-4f68-986c-2e487fc86ee0>,
        <https://data.amerigeoss.org/dataset/088f58ab-0516-40f6-8004-9d40a03096cb/resource/880c83a0-d093-401f-af33-41f4b134f9dd>,
        <https://data.amerigeoss.org/dataset/088f58ab-0516-40f6-8004-9d40a03096cb/resource/8b6347f4-f6e8-44be-9f04-8b457724a969>,
        <https://data.amerigeoss.org/dataset/088f58ab-0516-40f6-8004-9d40a03096cb/resource/8f8e367a-0537-44cb-8dde-2405c29e40b7>,
        <https://data.amerigeoss.org/dataset/088f58ab-0516-40f6-8004-9d40a03096cb/resource/b55f843b-3377-4b6c-b7b2-67bff740f39c>,
        <https://data.amerigeoss.org/dataset/088f58ab-0516-40f6-8004-9d40a03096cb/resource/e4a3321f-06cb-470c-a48d-8ddb9e947a8c> ;
    dcat:keyword "amerigeo",
        "amerigeoss",
        "ckan",
        "faang",
        "geo",
        "geoss",
        "immune-cells",
        "national",
        "north-america",
        "np108",
        "pbmc",
        "pig",
        "porcine",
        "porcine-leukocytes",
        "reference-transcriptomes",
        "rna-seq",
        "rna-sequencing",
        "scrna-seq",
        "single-cell",
        "single-cell-rna-sequencing",
        "transcriptome",
        "united-states" .

<https://data.amerigeoss.org/dataset/088f58ab-0516-40f6-8004-9d40a03096cb/resource/0512a6ad-0c61-430a-8ad2-9b79eafd625b> a dcat:Distribution ;
    dct:description "PBMC7_AllCells.h5Seurat.zip" ;
    dct:format "ZIP" ;
    dct:issued "2022-02-04T07:31:34.436240"^^xsd:dateTime ;
    dct:modified "2025-11-19T21:25:47.904217"^^xsd:dateTime ;
    dct:title "Zip File" ;
    dcat:accessURL <https://data.nal.usda.gov/system/files/PBMC7_AllCells.h5Seurat.zip> ;
    dcat:mediaType "application/zip" .

<https://data.amerigeoss.org/dataset/088f58ab-0516-40f6-8004-9d40a03096cb/resource/29ba57ad-b7e2-4825-936f-46156163471d> a dcat:Distribution ;
    dct:description "PBMC7_AllCells_UMAPcoord.csv" ;
    dct:format "CSV" ;
    dct:issued "2022-02-04T07:31:34.436261"^^xsd:dateTime ;
    dct:modified "2025-11-19T21:25:47.907241"^^xsd:dateTime ;
    dct:title "Comma Separated Values File" ;
    dcat:accessURL <https://data.nal.usda.gov/system/files/PBMC7_AllCells_UMAPcoord.csv> ;
    dcat:mediaType "text/csv" .

<https://data.amerigeoss.org/dataset/088f58ab-0516-40f6-8004-9d40a03096cb/resource/3ecd5b03-7aac-4669-b2e5-107aa2318368> a dcat:Distribution ;
    dct:description "UnfilteredGeneInfo.txt" ;
    dct:format "TEXT" ;
    dct:issued "2022-02-04T07:31:34.436286"^^xsd:dateTime ;
    dct:modified "2025-11-19T21:25:47.910579"^^xsd:dateTime ;
    dct:title "Text File" ;
    dcat:accessURL <https://data.nal.usda.gov/system/files/UnfilteredGeneInfo.txt> ;
    dcat:mediaType "text/plain" .

<https://data.amerigeoss.org/dataset/088f58ab-0516-40f6-8004-9d40a03096cb/resource/54cd0a40-4d60-417f-a546-eb408c3a642a> a dcat:Distribution ;
    dct:description "PBMC7_CD4only_tSNEcoord.csv" ;
    dct:format "CSV" ;
    dct:issued "2022-02-04T07:31:34.436266"^^xsd:dateTime ;
    dct:modified "2025-11-19T21:25:47.907955"^^xsd:dateTime ;
    dct:title "Comma Separated Values File" ;
    dcat:accessURL <https://data.nal.usda.gov/system/files/PBMC7_CD4only_tSNEcoord.csv> ;
    dcat:mediaType "text/csv" .

<https://data.amerigeoss.org/dataset/088f58ab-0516-40f6-8004-9d40a03096cb/resource/66ea6bbe-8cf9-471c-8449-2888109a951c> a dcat:Distribution ;
    dct:description "PBMC7_AllCells.zip" ;
    dct:format "ZIP" ;
    dct:issued "2022-02-04T07:31:34.436230"^^xsd:dateTime ;
    dct:modified "2025-11-19T21:25:47.903388"^^xsd:dateTime ;
    dct:title "Zip File" ;
    dcat:accessURL <https://data.nal.usda.gov/system/files/PBMC7_AllCells.zip> ;
    dcat:mediaType "application/zip" .

<https://data.amerigeoss.org/dataset/088f58ab-0516-40f6-8004-9d40a03096cb/resource/6a2a8452-5eb5-4f68-986c-2e487fc86ee0> a dcat:Distribution ;
    dct:description "PBMC7_CD4only_UMAPcoord.csv" ;
    dct:format "CSV" ;
    dct:issued "2022-02-04T07:31:34.436271"^^xsd:dateTime ;
    dct:modified "2025-11-19T21:25:47.908605"^^xsd:dateTime ;
    dct:title "Comma Separated Values File" ;
    dcat:accessURL <https://data.nal.usda.gov/system/files/PBMC7_CD4only_UMAPcoord.csv> ;
    dcat:mediaType "text/csv" .

<https://data.amerigeoss.org/dataset/088f58ab-0516-40f6-8004-9d40a03096cb/resource/880c83a0-d093-401f-af33-41f4b134f9dd> a dcat:Distribution ;
    dct:description "PBMC7_AllCells_tSNEcoord.csv" ;
    dct:format "CSV" ;
    dct:issued "2022-02-04T07:31:34.436257"^^xsd:dateTime ;
    dct:modified "2025-11-19T21:25:47.906453"^^xsd:dateTime ;
    dct:title "Comma Separated Values File" ;
    dcat:accessURL <https://data.nal.usda.gov/system/files/PBMC7_AllCells_tSNEcoord.csv> ;
    dcat:mediaType "text/csv" .

<https://data.amerigeoss.org/dataset/088f58ab-0516-40f6-8004-9d40a03096cb/resource/8b6347f4-f6e8-44be-9f04-8b457724a969> a dcat:Distribution ;
    dct:description "PBMC7_AllCells_PCAcoord.csv" ;
    dct:format "CSV" ;
    dct:issued "2022-02-04T07:31:34.436252"^^xsd:dateTime ;
    dct:modified "2025-11-19T21:25:47.905722"^^xsd:dateTime ;
    dct:title "Comma Separated Values File" ;
    dcat:accessURL <https://data.nal.usda.gov/system/files/PBMC7_AllCells_PCAcoord.csv> ;
    dcat:mediaType "text/csv" .

<https://data.amerigeoss.org/dataset/088f58ab-0516-40f6-8004-9d40a03096cb/resource/8f8e367a-0537-44cb-8dde-2405c29e40b7> a dcat:Distribution ;
    dct:description "PBMC7_GDonly_UMAPcoord.csv" ;
    dct:format "CSV" ;
    dct:issued "2022-02-04T07:31:34.436276"^^xsd:dateTime ;
    dct:modified "2025-11-19T21:25:47.909241"^^xsd:dateTime ;
    dct:title "Comma Separated Values File" ;
    dcat:accessURL <https://data.nal.usda.gov/system/files/PBMC7_GDonly_UMAPcoord.csv> ;
    dcat:mediaType "text/csv" .

<https://data.amerigeoss.org/dataset/088f58ab-0516-40f6-8004-9d40a03096cb/resource/b55f843b-3377-4b6c-b7b2-67bff740f39c> a dcat:Distribution ;
    dct:description "PBMC7_GDonly_tSNEcoord.csv" ;
    dct:format "CSV" ;
    dct:issued "2022-02-04T07:31:34.436281"^^xsd:dateTime ;
    dct:modified "2025-11-19T21:25:47.909883"^^xsd:dateTime ;
    dct:title "Comma Separated Values File" ;
    dcat:accessURL <https://data.nal.usda.gov/system/files/PBMC7_GDonly_tSNEcoord.csv> ;
    dcat:mediaType "text/csv" .

<https://data.amerigeoss.org/dataset/088f58ab-0516-40f6-8004-9d40a03096cb/resource/e4a3321f-06cb-470c-a48d-8ddb9e947a8c> a dcat:Distribution ;
    dct:description "PBMC7_AllCells_meta.csv" ;
    dct:format "CSV" ;
    dct:issued "2022-02-04T07:31:34.436247"^^xsd:dateTime ;
    dct:modified "2025-11-19T21:25:47.905003"^^xsd:dateTime ;
    dct:title "Comma Separated Values File" ;
    dcat:accessURL <https://data.nal.usda.gov/system/files/PBMC7_AllCells_meta.csv> ;
    dcat:mediaType "text/csv" .

<https://data.amerigeoss.org/organization/727dbdd5-3f98-4ac0-9d28-5e344558139b> a foaf:Agent ;
    foaf:name "US Migrating" .