@prefix dcat: <http://www.w3.org/ns/dcat#> .
@prefix dct: <http://purl.org/dc/terms/> .
@prefix foaf: <http://xmlns.com/foaf/0.1/> .
@prefix vcard: <http://www.w3.org/2006/vcard/ns#> .
@prefix xsd: <http://www.w3.org/2001/XMLSchema#> .

<https://data.amerigeoss.org/dataset/150d6794-519f-4532-9c2f-a73b510c0e64> a dcat:Dataset ;
    dct:description """Background
          Suppression Subtractive Hybridization PCR (SSH PCR) is a sophisticated cDNA subtraction method to enrich and isolate differentially expressed genes. Despite its popularity, the method has not been thoroughly studied for its practical efficacy and potential limitations.
        
        
          Results
          To determine the factors that influence the efficacy of SSH PCR, a theoretical model, under the assumption that cDNA hybridization follows the ideal second kinetic order, is proposed. The theoretical model suggests that the critical factor influencing the efficacy of SSH PCR is the concentration ratio (R) of a target gene between two cDNA preparations. It preferentially enriches "all or nothing" differentially expressed genes, of which R is infinite, and strongly favors the genes with large R. The theoretical predictions were validated by our experiments. In addition, the experiments revealed some practical limitations that are not obvious from the theoretical model. For effective enrichment of differentially expressed genes, it requires fractional concentration of a target gene to be more than 0.01% and concentration ratio to be more than 5 folds between two cDNA preparations.
        
        
          Conclusion
          Our research demonstrated theoretical and practical limitations of SSH PCR, which could be useful for its experimental design and interpretation.""" ;
    dct:identifier <https://healthdata.gov/api/views/xxpu-fi3d> ;
    dct:issued "2025-07-14"^^xsd:date ;
    dct:modified "2025-09-06"^^xsd:date ;
    dct:publisher <https://data.amerigeoss.org/organization/727dbdd5-3f98-4ac0-9d28-5e344558139b> ;
    dct:title "Efficacy of SSH PCR in isolating differentially expressed genes" ;
    dcat:contactPoint [ a vcard:Organization ;
            vcard:fn "NIH" ;
            vcard:hasEmail <mailto:info@nih.gov> ] ;
    dcat:distribution <https://data.amerigeoss.org/dataset/150d6794-519f-4532-9c2f-a73b510c0e64/resource/568e5591-3f8d-4ddf-b606-497a8e191198> ;
    dcat:keyword "AmeriGEO",
        "AmeriGEOSS",
        "CKAN",
        "GEO",
        "GEOSS",
        "National",
        "North America",
        "United States",
        "cdna-subtraction",
        "differential-expression",
        "gene-expression",
        "nih",
        "ssh-pcr" ;
    dcat:theme <%7BNIH%7D> .

<https://data.amerigeoss.org/dataset/150d6794-519f-4532-9c2f-a73b510c0e64/resource/568e5591-3f8d-4ddf-b606-497a8e191198> a dcat:Distribution ;
    dct:description "Visit the original government dataset for complete information, documentation, and data access." ;
    dct:format "HTML" ;
    dct:issued "2025-07-24T13:55:38.132400"^^xsd:dateTime ;
    dct:modified "2025-09-24T00:04:17.469396"^^xsd:dateTime ;
    dct:title "Official Government Data Source" ;
    dcat:accessURL <https://www.ncbi.nlm.nih.gov/pmc/articles/PMC115870/> ;
    dcat:mediaType "text/html" .

<https://data.amerigeoss.org/organization/727dbdd5-3f98-4ac0-9d28-5e344558139b> a foaf:Agent ;
    foaf:name "US Migrating" .