Acquisition of tolerance against oxidative damage in
type: Dataset
description: Background Living cells constantly sense and adapt to redox shifts by the induction of genes whose products act to maintain the cellular redox environment. In the eukaryote Saccharomyces cerevisiae, while stationary cells possess a degree of constitutive resistance towards oxidants, treatment of exponential phase cultures with sub-lethal stresses can lead to the transient induction of protection against subsequent lethal oxidant conditions. The sensors of oxidative stress and the corresponding transcription factors that activate gene expression under these conditions have not yet been completely identified. Results We report the role of SOD1, SOD2 and TPS1 genes (which encode the cytoplasmic Cu/Zn-superoxide dismutase, the mitochondrial Mn-isoform and trehalose-6-phosphate synthase, respectively) in the development of resistance to oxidative stress. In all experimental conditions, the cultures were divided into two parts, one was immediately submitted to severe stress (namely: exposure to H2O2, heat shock or ethanol stress) while the other was initially adapted to 40°C for 60 min. The deficiency in trehalose synthesis did not impair the acquisition of tolerance to H2O2, but this disaccharide played an essential role in tolerance against heat and ethanol stresses. We also verified that the presence of only one Sodp isoform was sufficient to improve cellular resistance to 5 mM H2O2. On the other hand, while the lack of Sod2p caused high cell sensitivity to ethanol and heat shock, the absence of Sod1p seemed to be beneficial to the process of acquisition of tolerance to these adverse conditions. The increase in oxidation-dependent fluorescence of crude extracts of sod1 mutant cells upon incubation at 40°C was approximately 2-fold higher than in sod2 and control strain extracts. Furthermore, in Western blots, we observed that sod mutants showed a different pattern of Hsp104p and Hsp26p expression also different from that in their control strain. Conclusions Trehalose se…
keyword: AmeriGEO
keyword: AmeriGEOSS
keyword: CKAN
keyword: GEO
keyword: GEOSS
keyword: National
keyword: North America
keyword: United States
keyword: nih
keyword: oxidative-stress
keyword: redox-regulation
keyword: saccharomyces-cerevisiae
keyword: stress-tolerance
issued: 2025-07-14
modified: 2025-09-06
theme: {NIH}
contact point: Organization
  fn: NIH

US Migrating
type: Agent

Official Government Data Source
type: Distribution
description: Visit the original government dataset for complete information, documentation, and data access.
media type: text/html
format: HTML
access URL: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC35392/
issued: 2025-07-24T13:46:41.104551
modified: 2025-09-24T17:09:50.858800