@prefix dcat: <http://www.w3.org/ns/dcat#> .
@prefix dct: <http://purl.org/dc/terms/> .
@prefix foaf: <http://xmlns.com/foaf/0.1/> .
@prefix vcard: <http://www.w3.org/2006/vcard/ns#> .
@prefix xsd: <http://www.w3.org/2001/XMLSchema#> .

<https://data.amerigeoss.org/dataset/2b0b405d-312b-4c19-80ce-1f4270c2c84d> a dcat:Dataset ;
    dct:description """The current study constitutes the first report of the metabolism of imidacloprid and acetamiprid in rainbow trout. Formation of respective metabolites 5-hydroxy-imidacloprid and N-desmethyl-acetamiprid was validated by analytical methods and demonstrated to be conserved across orders of biological organization in both microsomal and liver slice assays. Michaelis-Menten kinetics were determined for the microsomal conversion of imidacloprid to 5-hydroxy-imidacloprid in rainbow trout (Km=79.2 µM; Vmax=0.75 pmole/min/mg) and rat (Km=158.7 µM; Vmax=38.4 pmole/min/mg). Kinetics for the microsomal demethylation of acetamiprid to N-desmethyl-acetamiprid were determined in the rat (Km=70.9 µM; Vmax=4.10 pmoles/min/mg). N-desmethyl-acetamiprid was found in detectable but below quantifiable levels across the range of test concentrations which precluded a calculation of kinetic rate constants in rainbow trout (RBT). Ultimately, the formation of the metabolites 5-hydroxy-imidacloprid and N-desmethyl-acetamiprid was conserved across  RBT and rat species. 

This dataset is associated with the following publication:
Kolanczyk, R., M. Tapper, B. Sheedy, and J. Serrano. In vitro metabolism of imidacloprid and acetamiprid in rainbow trout and rat.   XENOBIOTICA. Taylor & Francis, Inc., Philadelphia, PA, USA, 50(7): 805-814, (2020).""" ;
    dct:identifier <https://doi.org/10.23719/1504449> ;
    dct:issued "2025-09-23T14:26:00.730677"^^xsd:dateTime ;
    dct:modified "2019-05-15"^^xsd:date ;
    dct:publisher <https://data.amerigeoss.org/organization/727dbdd5-3f98-4ac0-9d28-5e344558139b> ;
    dct:title "In Vitro Metabolism of Imidacloprid and Acetamiprid in Rainbow Trout and Rat - Kolanczyk_A-dv4j_SDMP_20190918" ;
    dcat:contactPoint [ a vcard:Organization ;
            vcard:fn "Richard Kolanczyk" ;
            vcard:hasEmail <mailto:kolanczyk.rick@epa.gov> ] ;
    dcat:distribution <https://data.amerigeoss.org/dataset/2b0b405d-312b-4c19-80ce-1f4270c2c84d/resource/e06af00e-a2b0-4cb8-9dde-fdbe75e49e70> ;
    dcat:keyword "AmeriGEO",
        "AmeriGEOSS",
        "CKAN",
        "GEO",
        "GEOSS",
        "National",
        "North America",
        "United States",
        "acetamiprid",
        "imidacloprid",
        "in-vitro",
        "liver-slice",
        "metabolism",
        "microsomes",
        "neonicotinoid",
        "rainbow-trout",
        "rat" .

<https://data.amerigeoss.org/dataset/2b0b405d-312b-4c19-80ce-1f4270c2c84d/resource/e06af00e-a2b0-4cb8-9dde-fdbe75e49e70> a dcat:Distribution ;
    dct:description "Imidacloprid%20acetamiprid%20trout%20vs%20rat%20data.xlsx" ;
    dct:format "XLS" ;
    dct:issued "2022-08-14T01:18:18.410703"^^xsd:dateTime ;
    dct:modified "2025-09-23T14:26:00.711688"^^xsd:dateTime ;
    dct:title "Imidacloprid acetamiprid trout vs rat data.xlsx" ;
    dcat:accessURL <https://pasteur.epa.gov/uploads/10.23719/1504449/Imidacloprid%20acetamiprid%20trout%20vs%20rat%20data.xlsx> ;
    dcat:mediaType "application/vnd.openxmlformats-officedocument.spreadsheetml.sheet" .

<https://data.amerigeoss.org/organization/727dbdd5-3f98-4ac0-9d28-5e344558139b> a foaf:Agent ;
    foaf:name "US Migrating" .