@prefix dcat: <http://www.w3.org/ns/dcat#> .
@prefix dct: <http://purl.org/dc/terms/> .
@prefix foaf: <http://xmlns.com/foaf/0.1/> .
@prefix vcard: <http://www.w3.org/2006/vcard/ns#> .
@prefix xsd: <http://www.w3.org/2001/XMLSchema#> .

<https://data.amerigeoss.org/dataset/1b06ce1e-6f46-45dc-bec2-de7704fdfcd5> a dcat:Dataset ;
    dct:description """<p>Primer3web version 4.0.0 allows users to pick PCR primers from a DNA sequence based on criteria including:</p>
<ul>
<li>
<p>oligonucleotide melting temperature, size, GC content, and primer-dimer possibilities,</p>
</li>
<li>
<p>PCR product size,</p>
</li>
<li>
<p>positional constraints within the source (template) sequence, and</p>
</li>
<li>
<p>possibilities for ectopic priming (amplifying the wrong sequence)</p>
</li>
<li>
<p>many other constraints.</p>
</li>
</ul>
<p>All of these criteria are user-specifiable as constraints, and some are specifiable as terms in an objective function that characterizes an optimal primer pair.</p>
<div><br>Resources in this dataset:</div><br><ul><li><p>Resource Title: Website Pointer for CottonGen Primer3.</p> <p>File Name: Web Page, url: <a href="https://www.cottongen.org/tools/primer3" target="_blank">https://www.cottongen.org/tools/primer3</a> </p></li></ul>""" ;
    dct:identifier "10113/AA23039" ;
    dct:issued "2025-09-24T03:58:28.793120"^^xsd:dateTime ;
    dct:modified "2024-02-13"^^xsd:date ;
    dct:publisher <https://data.amerigeoss.org/organization/727dbdd5-3f98-4ac0-9d28-5e344558139b> ;
    dct:title "CottonGen Primer3" ;
    dcat:contactPoint [ a vcard:Organization ;
            vcard:fn "Main, Dorrie" ;
            vcard:hasEmail <mailto:dorrie@wsu.edu> ] ;
    dcat:distribution <https://data.amerigeoss.org/dataset/1b06ce1e-6f46-45dc-bec2-de7704fdfcd5/resource/2b6d7412-6558-4f31-837c-72e97193e49e> ;
    dcat:keyword "AmeriGEO",
        "AmeriGEOSS",
        "CKAN",
        "GEO",
        "GEOSS",
        "National",
        "North America",
        "United States",
        "ars",
        "cottongen",
        "data-gov",
        "np301",
        "online-database",
        "primer3" .

<https://data.amerigeoss.org/dataset/1b06ce1e-6f46-45dc-bec2-de7704fdfcd5/resource/2b6d7412-6558-4f31-837c-72e97193e49e> a dcat:Distribution ;
    dct:format "HTML" ;
    dct:issued "2024-03-30T11:05:23.070486"^^xsd:dateTime ;
    dct:modified "2025-09-24T03:58:28.768718"^^xsd:dateTime ;
    dct:title "https://www.cottongen.org/tools/primer3" ;
    dcat:accessURL <https://www.cottongen.org/tools/primer3> ;
    dcat:mediaType "text/html" .

<https://data.amerigeoss.org/organization/727dbdd5-3f98-4ac0-9d28-5e344558139b> a foaf:Agent ;
    foaf:name "US Migrating" .