16S and ITS amplicon sequencing of reconstructed wetland soil metagenomes

Experiments tested if changes in soil microbiomes within reconstructed freshwater wetlands were associated with exposure to elevated Cu concentrations originating from immersed Micronized Copper Azole (MCA) treated wood stakes. Soil samples were collected for amplicon sequencing to quantify responses in microbial communities at 15 timepoints, spanning 2 simulated seasonal dry downs, for up to 678 days.

This dataset is associated with the following publication: Reichman, J., M. Johnson, P. Rygiewicz, B. Smith, M. Bollman, M. Storm, G. King, and C. Andersen. Focused Microbiome Shifts in Reconstructed Wetlands Correlated with Elevated Copper Concentrations Orginating from Micronized Copper Azole- Treated Wood. ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY. Society of Environmental Toxicology and Chemistry, Pensacola, FL, USA, 40(12): 3351-3368, (2021).

Data and Resources

Field Value
accessLevel public
bureauCode {020:00}
catalog_conformsTo https://project-open-data.cio.gov/v1.1/schema
identifier https://doi.org/10.23719/1522604
license https://pasteur.epa.gov/license/sciencehub-license.html
modified 2021-04-23
programCode {020:000}
publisher U.S. EPA Office of Research and Development (ORD)
publisher_hierarchy U.S. Government > U.S. Environmental Protection Agency > U.S. EPA Office of Research and Development (ORD)
references {https://doi.org/10.1002/etc.5219}
resource-type Dataset
source_datajson_identifier true
source_hash f51c1c1c5049376caa327aab5685c89807d0477b
source_schema_version 1.1
Groups
  • AmeriGEOSS
  • National Provider
  • North America
Tags
  • 16s-rrna-amplicon-sequencing
  • amerigeo
  • amerigeoss
  • ckan
  • engineered-nanomaterials
  • geo
  • geoss
  • its-rrna-amplicon-sequencing
  • microbial-communities
  • microbiome
  • micronized-copper-azole
  • national
  • north-america
  • united-states
  • wetlands
isopen False
license_id other-license-specified
license_title other-license-specified
maintainer Jay Reichman
maintainer_email reichman.jay@epa.gov
metadata_created 2025-11-22T10:15:44.080337
metadata_modified 2025-11-22T10:15:44.080340
notes Experiments tested if changes in soil microbiomes within reconstructed freshwater wetlands were associated with exposure to elevated Cu concentrations originating from immersed Micronized Copper Azole (MCA) treated wood stakes. Soil samples were collected for amplicon sequencing to quantify responses in microbial communities at 15 timepoints, spanning 2 simulated seasonal dry downs, for up to 678 days. This dataset is associated with the following publication: Reichman, J., M. Johnson, P. Rygiewicz, B. Smith, M. Bollman, M. Storm, G. King, and C. Andersen. Focused Microbiome Shifts in Reconstructed Wetlands Correlated with Elevated Copper Concentrations Orginating from Micronized Copper Azole- Treated Wood. ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY. Society of Environmental Toxicology and Chemistry, Pensacola, FL, USA, 40(12): 3351-3368, (2021).
num_resources 1
num_tags 15
title 16S and ITS amplicon sequencing of reconstructed wetland soil metagenomes