The objective of this investigation was to respond to a request for regional assistance for a comprehensive delineation of wetland habitats subject to jurisdiction under Section 404 of the Clean Water Act and all wetlands subject to National Park Service (NPS) procedures for implementing Director’s Order #77–1: Wetland Protection at three wetlands sites located on Thompson, Grape, and Peddocks Islands within Boston Harbor Islands National Park Area (BOHA). This work utilized the U.S Army Corps of Engineers (USACE) Interim Regional Supplement to the Corps of Engineers Wetland Delineation Manual: Northeast and Northcentral Region (January 2012) as the primary methodology for making wetland determinations in the field. A total of 3 palustrine wetlands encompassing 4.50 acres of land were identified and delineated from 14-15 October 2014 within the three islands area (Fig. 1). Observed wetland ecosystems were primarily the following habitats: palustrine, emergent, persistent, (PEM1); palustrine forested, broad leaved deciduous (PFO1); palustrine, unconsolidated bottom, mud (PUB3) These sites were specifically chosen by National Park Service (NPS) and US Forest Service Scientists (USFS) staff as research locations for an invasive plant removal study and this work was performed to identify the regulatory wetland boundaries and facilitate the acquisition of any compliance documents under Section 404 and DO #77.1 purview. All of the identified and mapped palustrine wetland systems encountered during the October 2014 investigation met both the U.S. Army Corps of Engineers criteria and the Cowardin et al. (1979) definitions of wetland habitat. No new riverine wetland systems were identified and mapped over the course of this investigation.