Conventional PCR results for detecting Tetracapsuloides bryosalmonae in fish tissue and water samples

We developed and validated conventional and quantitative real-time PCR assays for the detection of DNA from the myxozoan parasite Tetracapsuloides bryosalmonae, the causative agent of proliferative kidney disease in fish. Assays were tested on fish tissue and on field-collected water samples to assess diagnostic and environmental DNA capabilities. The specificity, sensitivity, and broad applicability of the present assays surpass previous methods for detecting T. bryosalmonae DNA from fish tissue and water samples.

Data and Resources

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modified 20200826
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publisher U.S. Geological Survey
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Groups
  • AmeriGEOSS
  • National Provider
  • North America
Tags
  • amerigeo
  • amerigeoss
  • biota
  • california
  • ckan
  • east-gallatin-river
  • environmental-dna
  • fish
  • geo
  • geoss
  • idaho
  • molecular-biology
  • montana
  • national
  • north-america
  • parasites
  • pcr
  • proliferative-kidney-disease
  • sypes-canyon
  • tetracapsuloides-bryosalmonae
  • united-states
  • usgs-594bedc5e4b062508e3850f0
  • wyoming
isopen False
license_id notspecified
license_title License not specified
maintainer Adam J Sepulveda
maintainer_email asepulveda@usgs.gov
metadata_created 2025-11-19T15:03:59.330368
metadata_modified 2025-11-19T15:03:59.330376
notes We developed and validated conventional and quantitative real-time PCR assays for the detection of DNA from the myxozoan parasite Tetracapsuloides bryosalmonae, the causative agent of proliferative kidney disease in fish. Assays were tested on fish tissue and on field-collected water samples to assess diagnostic and environmental DNA capabilities. The specificity, sensitivity, and broad applicability of the present assays surpass previous methods for detecting T. bryosalmonae DNA from fish tissue and water samples.
num_resources 2
num_tags 23
title Conventional PCR results for detecting Tetracapsuloides bryosalmonae in fish tissue and water samples