Coral microbiome preservation and extraction method comparison of samples collected in March and August 2018-raw data

The files in this this U.S. Geological Survey (USGS) data release (Kellogg and others, 2021) are the raw 16S ribosomal ribonucleic acid (rRNA) gene amplicon deoxyribonucleic acid (DNA) sequence files from 90 samples of tropical and cold-water corals, as well as sequence files from a mock community and extraction blanks for the kits used for DNA extraction. The mock community was sequenced in order to assess any biases in the sequencing technology, while extraction blanks were sequenced in order to identify any contaminants in the DNA extraction kits. The tropical coral samples (three species) were collected by permit (#FKNMS-2017-064) in March 2018 from a nursery in the Florida Keys National Marine Sanctuary. The cold-water coral samples (two species) were collected in August 2018 from two locations in the Atlantic Ocean.

Data and Resources

Field Value
accessLevel public
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identifier USGS:ba147df0-0de1-4f37-92f6-2712c0e21547
metadata_type geospatial
modified 20210923
old-spatial -81.45441, 24.661751, -75.16639, 34.9410185
publisher U.S. Geological Survey
publisher_hierarchy Department of the Interior > U.S. Geological Survey
resource-type Dataset
source_datajson_identifier true
source_hash 503bbb5b35858ca507aaa7953b000288182a4429
source_schema_version 1.1
spatial {"type": "Polygon", "coordinates": [[[-81.45441, 24.661751], [-81.45441, 34.9410185], [ -75.16639, 34.9410185], [ -75.16639, 24.661751], [-81.45441, 24.661751]]]}
theme {geospatial}
Groups
  • AmeriGEOSS
  • National Provider
  • North America
Tags
  • amerigeo
  • amerigeoss
  • atlantic-ocean
  • biota
  • ckan
  • coelenterates
  • florida-keys
  • geo
  • geoss
  • holocene
  • marine-biology
  • marine-geology
  • national
  • north-america
  • oceans
  • pamlico-canyon
  • richardson-hills
  • summerland-key
  • united-states
  • usgs-ba147df0-0de1-4f37-92f6-2712c0e21547
isopen False
license_id notspecified
license_title License not specified
maintainer Christina A. Kellogg
maintainer_email ckellogg@usgs.gov
metadata_created 2025-11-21T01:00:52.073701
metadata_modified 2025-11-21T01:00:52.073707
notes The files in this this U.S. Geological Survey (USGS) data release (Kellogg and others, 2021) are the raw 16S ribosomal ribonucleic acid (rRNA) gene amplicon deoxyribonucleic acid (DNA) sequence files from 90 samples of tropical and cold-water corals, as well as sequence files from a mock community and extraction blanks for the kits used for DNA extraction. The mock community was sequenced in order to assess any biases in the sequencing technology, while extraction blanks were sequenced in order to identify any contaminants in the DNA extraction kits. The tropical coral samples (three species) were collected by permit (#FKNMS-2017-064) in March 2018 from a nursery in the Florida Keys National Marine Sanctuary. The cold-water coral samples (two species) were collected in August 2018 from two locations in the Atlantic Ocean.
num_resources 2
num_tags 20
title Coral microbiome preservation and extraction method comparison of samples collected in March and August 2018-raw data