CottonGen Primer3

Primer3web version 4.0.0 allows users to pick PCR primers from a DNA sequence based on criteria including:

oligonucleotide melting temperature, size, GC content, and primer-dimer possibilities,

PCR product size,

positional constraints within the source (template) sequence, and

possibilities for ectopic priming (amplifying the wrong sequence)

many other constraints.

All of these criteria are user-specifiable as constraints, and some are specifiable as terms in an objective function that characterizes an optimal primer pair.

Data and Resources

Field Value
accessLevel public
bureauCode {005:18}
catalog_@context https://project-open-data.cio.gov/v1.1/schema/catalog.jsonld
catalog_conformsTo https://project-open-data.cio.gov/v1.1/schema
catalog_describedBy https://project-open-data.cio.gov/v1.1/schema/catalog.json
identifier 4a131184-c0cb-4ec0-8429-b6e26c5596b9
license https://creativecommons.org/publicdomain/zero/1.0/
modified 2022-01-31
programCode {005:040}
publisher Agricultural Research Service
resource-type Dataset
source_datajson_identifier true
source_hash 50bead97c6ef3ac21873cf82444670ab62e08dae
source_schema_version 1.1
Groups
  • AmeriGEOSS
  • National Provider
  • North America
Tags
  • amerigeo
  • amerigeoss
  • ckan
  • cottongen
  • geo
  • geoss
  • national
  • north-america
  • np301
  • primer3
  • united-states
isopen True
license_id cc-zero
license_title Creative Commons CCZero
license_url http://www.opendefinition.org/licenses/cc-zero
maintainer Main, Dorrie
maintainer_email dorrie@wsu.edu
metadata_created 2025-11-22T19:46:53.759422
metadata_modified 2025-11-22T19:46:53.759428
notes <p>Primer3web version 4.0.0 allows users to pick PCR primers from a DNA sequence based on criteria including:</p> <ul> <li> <p>oligonucleotide melting temperature, size, GC content, and primer-dimer possibilities,</p> </li> <li> <p>PCR product size,</p> </li> <li> <p>positional constraints within the source (template) sequence, and</p> </li> <li> <p>possibilities for ectopic priming (amplifying the wrong sequence)</p> </li> <li> <p>many other constraints.</p> </li> </ul> <p>All of these criteria are user-specifiable as constraints, and some are specifiable as terms in an objective function that characterizes an optimal primer pair.</p>
num_resources 1
num_tags 11
title CottonGen Primer3