Data from: Detection and differentiation of low virulence and virulent Orthoavulavirus javaense using a molecular beacon with RT-LAMP

This dataset accompanies the manuscript Detection and differentiation of low virulence and virulent Orthoavulavirus javaense using a molecular beacon with RT-LAMP | Scientific Reports (nature.com) published on 08/05/2024. The Orthoavulavirus javaense also called as Newcastle Disease virus is the causative of Newcastle disease a significant problem for the poultry industry. Virulent strains of this virus can cause extensive mortality and economic loss to the poultry industry. Current diagnostic methods rely on polymerase chain reaction targeting the fusion gene of this virus and this method is not only expensive and time consuming, but is also not field applicable. The dataset reported here and the manuscript published describe an assay that can be applied to field diagnosis of NDV via a colorimetric and fluorometric assay. The MB-RT-LAMP assay enables rapid detection of viruses from field samples without the need for RNA isolation and also enable rapid differentiation of virulent vs low virulence viruses. The data provided here demonstrate the sequence analysis undertaken to develop the MB-RT-LAMP primers and probes, the supporting data show the conditions tested to identify optimal parameters.

Data and Resources

Field Value
accessLevel public
accrualPeriodicity R/P1D
bureauCode {005:18}
catalog_@context https://project-open-data.cio.gov/v1.1/schema/catalog.jsonld
catalog_conformsTo https://project-open-data.cio.gov/v1.1/schema
catalog_describedBy https://project-open-data.cio.gov/v1.1/schema/catalog.json
identifier 10.15482/USDA.ADC/26792542.v1
license https://creativecommons.org/licenses/by/4.0/
modified 2025-06-03
programCode {005:040}
publisher Agricultural Research Service
resource-type Dataset
source_datajson_identifier true
source_hash f2e9e2208da969cf1a568bc64560d7266c0f6f5478af2e8ca1d060f59db18425
source_schema_version 1.1
temporal 2023-03-01/2024-08-05
Groups
  • AmeriGEOSS
  • National Provider
  • North America
Tags
  • AmeriGEO
  • AmeriGEOSS
  • CKAN
  • GEO
  • GEOSS
  • National
  • North America
  • United States
  • diagnostic-assay
  • loop-mediated-isothermal-amplification
  • newcastle-disease-virus
isopen True
license_id cc-by
license_title Creative Commons Attribution
license_url http://www.opendefinition.org/licenses/cc-by
maintainer Bakre, Abhijeet A.
maintainer_email abhijeet.bakre@usda.gov
metadata_created 2025-09-29T15:29:23.350431
metadata_modified 2025-09-29T15:29:23.350437
notes <p>This dataset accompanies the manuscript <a href="https://www.nature.com/articles/s41598-024-68816-7" target="_blank">Detection and differentiation of low virulence and virulent Orthoavulavirus javaense using a molecular beacon with RT-LAMP | Scientific Reports (nature.com)</a> published on 08/05/2024. The Orthoavulavirus javaense also called as Newcastle Disease virus is the causative of Newcastle disease a significant problem for the poultry industry. Virulent strains of this virus can cause extensive mortality and economic loss to the poultry industry. Current diagnostic methods rely on polymerase chain reaction targeting the fusion gene of this virus and this method is not only expensive and time consuming, but is also not field applicable. The dataset reported here and the manuscript published describe an assay that can be applied to field diagnosis of NDV via a colorimetric and fluorometric assay. The MB-RT-LAMP assay enables rapid detection of viruses from field samples without the need for RNA isolation and also enable rapid differentiation of virulent vs low virulence viruses. The data provided here demonstrate the sequence analysis undertaken to develop the MB-RT-LAMP primers and probes, the supporting data show the conditions tested to identify optimal parameters.</p>
num_resources 31
num_tags 11
title Data from: Detection and differentiation of low virulence and virulent Orthoavulavirus javaense using a molecular beacon with RT-LAMP