Data from: Reference transcriptomics of porcine peripheral immune cells created through bulk and single-cell RNA sequencing

This dataset contains files reconstructing single-cell data presented in 'Reference transcriptomics of porcine peripheral immune cells created through bulk and single-cell RNA sequencing' by Herrera-Uribe & Wiarda et al. 2021. Samples of peripheral blood mononuclear cells (PBMCs) were collected from seven pigs and processed for single-cell RNA sequencing (scRNA-seq) in order to provide a reference annotation of porcine immune cell transcriptomics at enhanced, single-cell resolution. Analysis of single-cell data allowed identification of 36 cell clusters that were further classified into 13 cell types, including monocytes, dendritic cells, B cells, antibody-secreting cells, numerous populations of T cells, NK cells, and erythrocytes. Files may be used to reconstruct the data as presented in the manuscript, allowing for individual query by other users. Scripts for original data analysis are available at https://github.com/USDA-FSEPRU/PorcinePBMCs_bulkRNAseq_scRNAseq. Raw data are available at https://www.ebi.ac.uk/ena/browser/view/PRJEB43826. Funding for this dataset was also provided by NRSP8: National Animal Genome Research Program (https://www.nimss.org/projects/view/mrp/outline/18464).

Data and Resources

Field Value
accessLevel public
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identifier 673fd36c-e9a0-4522-b6f1-cb68c54df85b
license https://creativecommons.org/publicdomain/zero/1.0/
modified 2022-01-05
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programCode {005:040}
publisher Agricultural Research Service
resource-type Dataset
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Groups
  • AmeriGEOSS
  • National Provider
  • North America
Tags
  • amerigeo
  • amerigeoss
  • ckan
  • faang
  • geo
  • geoss
  • immune-cells
  • national
  • north-america
  • np108
  • pbmc
  • pig
  • porcine
  • porcine-leukocytes
  • reference-transcriptomes
  • rna-seq
  • rna-sequencing
  • scrna-seq
  • single-cell
  • single-cell-rna-sequencing
  • transcriptome
  • united-states
isopen True
license_id cc-zero
license_title Creative Commons CCZero
license_url http://www.opendefinition.org/licenses/cc-zero
maintainer Loving, Crystal
maintainer_email crystal.loving@usda.gov
metadata_created 2025-11-19T21:25:47.930609
metadata_modified 2025-11-19T21:25:47.930616
notes <p>This dataset contains files reconstructing single-cell data presented in &#039;Reference transcriptomics of porcine peripheral immune cells created through bulk and single-cell RNA sequencing&#039; by Herrera-Uribe & Wiarda et al. 2021. Samples of peripheral blood mononuclear cells (PBMCs) were collected from seven pigs and processed for single-cell RNA sequencing (scRNA-seq) in order to provide a reference annotation of porcine immune cell transcriptomics at enhanced, single-cell resolution. Analysis of single-cell data allowed identification of 36 cell clusters that were further classified into 13 cell types, including monocytes, dendritic cells, B cells, antibody-secreting cells, numerous populations of T cells, NK cells, and erythrocytes. Files may be used to reconstruct the data as presented in the manuscript, allowing for individual query by other users. Scripts for original data analysis are available at <a href="https://github.com/USDA-FSEPRU/PorcinePBMCs_bulkRNAseq_scRNAseq">https://github.com/USDA-FSEPRU/PorcinePBMCs_bulkRNAseq_scRNAseq</a>. Raw data are available at <a href="https://www.ebi.ac.uk/ena/browser/view/PRJEB43826">https://www.ebi.ac.uk/ena/browser/view/PRJEB43826</a>.</p> <p>Funding for this dataset was also provided by NRSP8: National Animal Genome Research Program (<a href="https://www.nimss.org/projects/view/mrp/outline/18464">https://www.nimss.org/projects/view/mrp/outline/18464</a>).</p>
num_resources 11
num_tags 22
title Data from: Reference transcriptomics of porcine peripheral immune cells created through bulk and single-cell RNA sequencing