Dataset from HDX-MS Studies of IgG1 Glycoforms and Their Interactions with the Fc(gamma)RIa (CD64) Receptor

This database gives hydrogen-deuterium exchange mass spectrometry (HDX-MS) data from measurements of three purified IgG1 glycoform samples, predominantly G0F, G2F, and SAF, in isolation and in complexation with the high-affinity receptor, Fc(gamma)RIa (CD64). The IgG1 antibody used in this study, aIL8hFc, is a murine-human chimeric IgG1, which inhibits IL-8 binding to human neutrophils. The working datasets comprise 17,750 deuterium uptake measurements in peptides from the receptor and the light and heavy chains of aIL8hFc obtained during 7 experiments, each comprising three runs of six exchange times. The data illuminate on the hydrogen exchange dynamics in isolated receptor, isolated IgG1 glycoforms, and receptor-IgG1 complexes.

Data and Resources

Field Value
accessLevel public
bureauCode {006:55}
catalog_@context https://project-open-data.cio.gov/v1.1/schema/data.json
catalog_conformsTo https://project-open-data.cio.gov/v1.1/schema
catalog_describedBy https://project-open-data.cio.gov/v1.1/schema/catalog.json
identifier ark:/88434/mds2-2365
issued 2021-06-03
language {en}
license https://www.nist.gov/open/license
modified 2021-02-17 00:00:00
programCode {006:045}
publisher National Institute of Standards and Technology
resource-type Dataset
source_datajson_identifier true
source_hash 419d5697a93966dc394be8ca03163a62a7313ffa
source_schema_version 1.1
theme {Bioscience:Proteomics,"Chemistry:Analytical chemistry",Bioscience:Biomaterials,"Chemistry:Molecular characterization",Health:Pharmaceuticals,Manufacturing:Biomanufacturing,"Materials:Materials characterization"}
Groups
  • AmeriGEOSS
  • National Provider
  • North America
Tags
  • amerigeo
  • amerigeoss
  • antibody-receptor-interaction
  • chromatography
  • ckan
  • geo
  • geoss
  • glycosylation
  • hdx-ms
  • hydrogen-deuterium-exchange
  • mass-spectrometry
  • monoclonal-antibody
  • national
  • north-america
  • peptide
  • precision
  • protein
  • proteolysis
  • proteomics
  • receptor
  • united-states
isopen False
license_id other-license-specified
license_title other-license-specified
maintainer Jeffrey W. Hudgens
maintainer_email jeffrey.hudgens@nist.gov
metadata_created 2025-11-22T22:58:50.972766
metadata_modified 2025-11-22T22:58:50.972771
notes This database gives hydrogen-deuterium exchange mass spectrometry (HDX-MS) data from measurements of three purified IgG1 glycoform samples, predominantly G0F, G2F, and SAF, in isolation and in complexation with the high-affinity receptor, Fc(gamma)RIa (CD64). The IgG1 antibody used in this study, aIL8hFc, is a murine-human chimeric IgG1, which inhibits IL-8 binding to human neutrophils. The working datasets comprise 17,750 deuterium uptake measurements in peptides from the receptor and the light and heavy chains of aIL8hFc obtained during 7 experiments, each comprising three runs of six exchange times. The data illuminate on the hydrogen exchange dynamics in isolated receptor, isolated IgG1 glycoforms, and receptor-IgG1 complexes.
num_resources 37
num_tags 21
title Dataset from HDX-MS Studies of IgG1 Glycoforms and Their Interactions with the Fc(gamma)RIa (CD64) Receptor