ECOHAB: Kamykowski_D- Florida Process Cruise 1998-11-16 to 1998-11-19 Karenia brevis counts, biochemistry and behavior (NCEI Accession 0000534)

Water bottle samples were collected from selected depths (variable with station) at 14 stations in the study area. Coulter counts in the 14-28 um size class were routinely determined for comparison with the microscope counts. Water samples were filtered through 40 um Nytex mesh and centrifuged for 10 minutes at 1200 x g to isolate Karenia brevis. Pellets were frozen for subsequent analysis (pg cell-1) of total lipid, neutral lipid, free amino acids, protein and RNA. The biochemical determinations were performed within two weeks of collection at Kamykowski's NCSU laboratory. Aliquots were passed through 0.4 um filters; the filters were placed in 90% acetone for subsequent analysis of chlorophyll and the supernatant was analyzed for nitrate.

Data and Resources

Field Value
access_constraints ["Cite as: Kamykowski, Daniel (2001). ECOHAB: Kamykowski_D- Florida Process Cruise 1998-11-16 to 1998-11-19 Karenia brevis counts, biochemistry and behavior (NCEI Accession 0000534). [indicate subset used]. NOAA National Centers for Environmental Information. Dataset. https://www.ncei.noaa.gov/archive/accession/0000534. Accessed [date].", "Use liability: NOAA and NCEI cannot provide any warranty as to the accuracy, reliability, or completeness of furnished data. Users assume responsibility to determine the usability of these data. The user is responsible for the results of any application of this data for other than its intended purpose."]
bbox-east-long -86.22
bbox-north-lat 30.23
bbox-south-lat 30.22
bbox-west-long -86.29
contact-email ncei.info@noaa.gov
coupled-resource []
dataset-reference-date [{"type": "publication", "value": "2001-08-07"}]
frequency-of-update asNeeded
graphic-preview-description Graphic not available.
graphic-preview-file https://www.ncei.noaa.gov/access/metadata/landing-page/bin/gfx?id=gov.noaa.nodc:0000534
graphic-preview-type PNG
guid gov.noaa.nodc:0000534
licence ["accessLevel: Public"]
metadata-date 2022-11-04T11:33:50Z
metadata-language eng
metadata_type geospatial
old-spatial {"type": "Polygon", "coordinates": [[[-86.29, 30.22], [-86.22, 30.22], [-86.22, 30.23], [-86.29, 30.23], [-86.29, 30.22]]]}
progress completed
resource-type dataset
responsible-party [{"name": "", "roles": ["pointOfContact"]}]
spatial {"type": "Polygon", "coordinates": [[[-86.29, 30.22], [-86.22, 30.22], [-86.22, 30.23], [-86.29, 30.23], [-86.29, 30.22]]]}
spatial_harvester true
temporal-extent-begin 1999-09-23
temporal-extent-end 1999-09-30
Groups
  • AmeriGEOSS
  • National Provider
  • North America
Tags
  • AmeriGEO
  • AmeriGEOSS
  • CKAN
  • GEO
  • GEOSS
  • National
  • North America
  • United States
  • abundance
  • algae
  • aquatic
  • chemistry
  • destin beach area
  • distribution
  • dynamic
  • florida panhandle
  • marine
  • oceanography
  • organic
  • plankton
  • population
  • saltwater
  • statistics
isopen False
metadata_created 2025-09-24T16:15:58.155513
metadata_modified 2025-09-24T16:15:58.155521
notes Water bottle samples were collected from selected depths (variable with station) at 14 stations in the study area. Coulter counts in the 14-28 um size class were routinely determined for comparison with the microscope counts. Water samples were filtered through 40 um Nytex mesh and centrifuged for 10 minutes at 1200 x g to isolate Karenia brevis. Pellets were frozen for subsequent analysis (pg cell-1) of total lipid, neutral lipid, free amino acids, protein and RNA. The biochemical determinations were performed within two weeks of collection at Kamykowski's NCSU laboratory. Aliquots were passed through 0.4 um filters; the filters were placed in 90% acetone for subsequent analysis of chlorophyll and the supernatant was analyzed for nitrate.
num_resources 13
num_tags 23
title ECOHAB: Kamykowski_D- Florida Process Cruise 1998-11-16 to 1998-11-19 Karenia brevis counts, biochemistry and behavior (NCEI Accession 0000534)