Estimating Hepatotoxic Doses Using High-content Imaging in Primary Hepatocytes

This repository contains the necessary data, python source code and jupyter notebooks to reproduce the results from our manuscript, "Estimating Hepatotoxic Doses Using High-content Imaging in Primary Hepatocytes." Using in vitro data to estimate point of departure (POD) values is an important component of new approach method (NAM)-based chemical risk assessments. In this case study we evaluated a NAM for hepatotoxicity based on rat primary hepatocytes, high-content imaging (HCI) and in vitro to in vivo extrapolation (IVIVE).

This dataset is associated with the following publication: Shah, I., T. Antonijevic, B. Chambers, J. Harrill, and R. Thomas. Estimating Hepatotoxic Doses Using High-content Imaging in Primary Hepatocytes. TOXICOLOGICAL SCIENCES. Society of Toxicology, RESTON, VA, 183(2): 285-301, (2021).

Data and Resources

Field Value
accessLevel public
bureauCode {020:00}
catalog_conformsTo https://project-open-data.cio.gov/v1.1/schema
identifier https://doi.org/10.23719/1520925
license https://pasteur.epa.gov/license/sciencehub-license.html
modified 2020-09-09
programCode {020:095}
publisher U.S. EPA Office of Research and Development (ORD)
publisher_hierarchy U.S. Government > U.S. Environmental Protection Agency > U.S. EPA Office of Research and Development (ORD)
references {https://doi.org/10.1093/toxsci/kfab091}
resource-type Dataset
source_datajson_identifier true
source_hash 0bd2f8e80b0b06d63c9471ec37d2115c4f7ce02e
source_schema_version 1.1
Groups
  • AmeriGEOSS
  • National Provider
  • North America
Tags
  • AmeriGEO
  • AmeriGEOSS
  • CKAN
  • GEO
  • GEOSS
  • National
  • North America
  • United States
  • hepatotoxicity
  • high-content-imaging
  • new-approach-methodologies
  • pbtk
  • point-of-departure
  • repeat-dose-toxicity
  • tipping-points
  • virtual-embryo
  • virtual-liver
  • virtual-tissues
isopen False
license_id other-license-specified
license_title other-license-specified
maintainer Thomas Knudsen
maintainer_email knudsen.thomas@epa.gov
metadata_created 2025-09-24T00:29:26.567173
metadata_modified 2025-09-24T00:29:26.567180
notes This repository contains the necessary data, python source code and jupyter notebooks to reproduce the results from our manuscript, "Estimating Hepatotoxic Doses Using High-content Imaging in Primary Hepatocytes." Using in vitro data to estimate point of departure (POD) values is an important component of new approach method (NAM)-based chemical risk assessments. In this case study we evaluated a NAM for hepatotoxicity based on rat primary hepatocytes, high-content imaging (HCI) and in vitro to in vivo extrapolation (IVIVE). This dataset is associated with the following publication: Shah, I., T. Antonijevic, B. Chambers, J. Harrill, and R. Thomas. Estimating Hepatotoxic Doses Using High-content Imaging in Primary Hepatocytes. TOXICOLOGICAL SCIENCES. Society of Toxicology, RESTON, VA, 183(2): 285-301, (2021).
num_resources 1
num_tags 18
title Estimating Hepatotoxic Doses Using High-content Imaging in Primary Hepatocytes