Experimental PCR Data on Soil DNA Extracts

Bacillus species and B. anthracis presence/absence data were determined in 4,770 soil samples collected across the contiguous United States, in cooperation with the U.S. Environmental Protection Agency (EPA). Polymerase Chain Reaction (PCR) data for Bacillus species and B. anthracis rpoB gene PCR amplicon detection were reported as non-detect (n), low (l), medium (m), and high (h). Results for both pag and lef genes of the pX01 plasmid were reported by the University of South Florida's Center for Biological Defense. This data was recorded as negative or positive for each of the genes and included the following combinations: neg/neg, pos/neg, neg/pos, and pos/pos. Data for the pX02 plasmid were recorded as negative (neg) or positive (pos).

Data and Resources

Field Value
accessLevel public
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identifier USGS:dceca56e-1ae9-4d7d-a435-ac978e1c9b49
metadata_type geospatial
modified 20201013
old-spatial -124.4019, 25.1376, -67.5201, 48.9835
publisher U.S. Geological Survey
publisher_hierarchy Department of the Interior > U.S. Geological Survey
resource-type Dataset
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spatial {"type": "Polygon", "coordinates": [[[-124.4019, 25.1376], [-124.4019, 48.9835], [ -67.5201, 48.9835], [ -67.5201, 25.1376], [-124.4019, 25.1376]]]}
theme {geospatial}
Groups
  • AmeriGEOSS
  • National Provider
  • North America
Tags
  • absence
  • amerigeo
  • amerigeoss
  • bacillus-anthracis
  • biota
  • ckan
  • environment
  • environmental-health-human
  • francisella-tularensis
  • geo
  • geochemistry
  • geoscientificinformation
  • geoss
  • health
  • lef
  • national
  • north-america
  • pag
  • pcr
  • presence
  • sediment
  • soil
  • soil-sciences
  • subsoil
  • united-states
  • usgs-dceca56e-1ae9-4d7d-a435-ac978e1c9b49
  • yersinia-pestis
isopen False
license_id notspecified
license_title License not specified
maintainer Dale Griffin
maintainer_email dgriffin@usgs.gov
metadata_created 2025-11-20T16:41:19.565065
metadata_modified 2025-11-20T16:41:19.565069
notes Bacillus species and B. anthracis presence/absence data were determined in 4,770 soil samples collected across the contiguous United States, in cooperation with the U.S. Environmental Protection Agency (EPA). Polymerase Chain Reaction (PCR) data for Bacillus species and B. anthracis rpoB gene PCR amplicon detection were reported as non-detect (n), low (l), medium (m), and high (h). Results for both pag and lef genes of the pX01 plasmid were reported by the University of South Florida's Center for Biological Defense. This data was recorded as negative or positive for each of the genes and included the following combinations: neg/neg, pos/neg, neg/pos, and pos/pos. Data for the pX02 plasmid were recorded as negative (neg) or positive (pos).
num_resources 2
num_tags 27
title Experimental PCR Data on Soil DNA Extracts