In Vitro Metabolism of Imidacloprid and Acetamiprid in Rainbow Trout and Rat - Kolanczyk_A-dv4j_SDMP_20190918

The current study constitutes the first report of the metabolism of imidacloprid and acetamiprid in rainbow trout. Formation of respective metabolites 5-hydroxy-imidacloprid and N-desmethyl-acetamiprid was validated by analytical methods and demonstrated to be conserved across orders of biological organization in both microsomal and liver slice assays. Michaelis-Menten kinetics were determined for the microsomal conversion of imidacloprid to 5-hydroxy-imidacloprid in rainbow trout (Km=79.2 µM; Vmax=0.75 pmole/min/mg) and rat (Km=158.7 µM; Vmax=38.4 pmole/min/mg). Kinetics for the microsomal demethylation of acetamiprid to N-desmethyl-acetamiprid were determined in the rat (Km=70.9 µM; Vmax=4.10 pmoles/min/mg). N-desmethyl-acetamiprid was found in detectable but below quantifiable levels across the range of test concentrations which precluded a calculation of kinetic rate constants in rainbow trout (RBT). Ultimately, the formation of the metabolites 5-hydroxy-imidacloprid and N-desmethyl-acetamiprid was conserved across RBT and rat species.

This dataset is associated with the following publication: Kolanczyk, R., M. Tapper, B. Sheedy, and J. Serrano. In vitro metabolism of imidacloprid and acetamiprid in rainbow trout and rat. XENOBIOTICA. Taylor & Francis, Inc., Philadelphia, PA, USA, 50(7): 805-814, (2020).

Data and Resources

Field Value
accessLevel public
bureauCode {020:00}
catalog_conformsTo https://project-open-data.cio.gov/v1.1/schema
identifier https://doi.org/10.23719/1504449
license https://pasteur.epa.gov/license/sciencehub-license.html
modified 2019-05-15
programCode {020:000}
publisher U.S. EPA Office of Research and Development (ORD)
publisher_hierarchy U.S. Government > U.S. Environmental Protection Agency > U.S. EPA Office of Research and Development (ORD)
references {https://doi.org/10.1080/00498254.2019.1694197}
resource-type Dataset
source_datajson_identifier true
source_hash 9206d390b0f2775eeebf70efec66d290804da7c5
source_schema_version 1.1
Groups
  • AmeriGEOSS
  • National Provider
  • North America
Tags
  • AmeriGEO
  • AmeriGEOSS
  • CKAN
  • GEO
  • GEOSS
  • National
  • North America
  • United States
  • acetamiprid
  • imidacloprid
  • in-vitro
  • liver-slice
  • metabolism
  • microsomes
  • neonicotinoid
  • rainbow-trout
  • rat
isopen False
license_id other-license-specified
license_title other-license-specified
maintainer Richard Kolanczyk
maintainer_email kolanczyk.rick@epa.gov
metadata_created 2025-09-23T14:26:00.730677
metadata_modified 2025-09-23T14:26:00.730683
notes The current study constitutes the first report of the metabolism of imidacloprid and acetamiprid in rainbow trout. Formation of respective metabolites 5-hydroxy-imidacloprid and N-desmethyl-acetamiprid was validated by analytical methods and demonstrated to be conserved across orders of biological organization in both microsomal and liver slice assays. Michaelis-Menten kinetics were determined for the microsomal conversion of imidacloprid to 5-hydroxy-imidacloprid in rainbow trout (Km=79.2 µM; Vmax=0.75 pmole/min/mg) and rat (Km=158.7 µM; Vmax=38.4 pmole/min/mg). Kinetics for the microsomal demethylation of acetamiprid to N-desmethyl-acetamiprid were determined in the rat (Km=70.9 µM; Vmax=4.10 pmoles/min/mg). N-desmethyl-acetamiprid was found in detectable but below quantifiable levels across the range of test concentrations which precluded a calculation of kinetic rate constants in rainbow trout (RBT). Ultimately, the formation of the metabolites 5-hydroxy-imidacloprid and N-desmethyl-acetamiprid was conserved across RBT and rat species. This dataset is associated with the following publication: Kolanczyk, R., M. Tapper, B. Sheedy, and J. Serrano. In vitro metabolism of imidacloprid and acetamiprid in rainbow trout and rat. XENOBIOTICA. Taylor & Francis, Inc., Philadelphia, PA, USA, 50(7): 805-814, (2020).
num_resources 1
num_tags 17
title In Vitro Metabolism of Imidacloprid and Acetamiprid in Rainbow Trout and Rat - Kolanczyk_A-dv4j_SDMP_20190918