International Space Station conditions alter genomics proteomics and metabolomics in Aspergillus nidulans

The first global genomic proteomic and secondary metabolomic characterization of the filamentous fungus Aspergillus nidulans following growth onboard the International Space Station (ISS) is reported. The investigation included the A. nidulans wild-type and three mutant strains two of which were genetically engineered to enhance secondary metabolite production. Whole genome sequencing revealed that ISS conditions altered the A. nidulans genome in specific regions. In strain CW12001 which features overexpression of the secondary metabolite global regulator laeA ISS conditions induced the loss of the laeA stop codon. Differential expression of proteins involved in stress response carbohydrate metabolic processes and secondary metabolite biosynthesis was also observed. ISS conditions significantly decreased prenyl xanthone production in the wild-type strain and increased asperthecin production in LO1362 and CW12001 which are deficient in a major DNA repair mechanism. These data provide valuable insights into the adaptation mechanism of A. nidulans to spacecraft environments.

Data and Resources

Field Value
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identifier nasa_genelab_GLDS-233_fq84-y35b
issued 2021-05-21
landingPage https://data.nasa.gov/dataset/international-space-station-conditions-alter-genomics-proteomics-and-metabolomics-in-asper
license http://www.usa.gov/publicdomain/label/1.0/
modified 2025-04-23
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publisher National Aeronautics and Space Administration
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Groups
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  • National Provider
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  • AmeriGEOSS
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  • GEO
  • GEOSS
  • National
  • North America
  • United States
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  • library-construction
  • nucleic-acid-extraction
  • nucleic-acid-sequencing
  • sample-collection
  • sample-treatment
  • spaceflight
  • strain
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metadata_created 2025-09-25T01:10:36.215489
metadata_modified 2025-09-25T01:10:36.215499
notes The first global genomic proteomic and secondary metabolomic characterization of the filamentous fungus Aspergillus nidulans following growth onboard the International Space Station (ISS) is reported. The investigation included the A. nidulans wild-type and three mutant strains two of which were genetically engineered to enhance secondary metabolite production. Whole genome sequencing revealed that ISS conditions altered the A. nidulans genome in specific regions. In strain CW12001 which features overexpression of the secondary metabolite global regulator laeA ISS conditions induced the loss of the laeA stop codon. Differential expression of proteins involved in stress response carbohydrate metabolic processes and secondary metabolite biosynthesis was also observed. ISS conditions significantly decreased prenyl xanthone production in the wild-type strain and increased asperthecin production in LO1362 and CW12001 which are deficient in a major DNA repair mechanism. These data provide valuable insights into the adaptation mechanism of A. nidulans to spacecraft environments.
num_resources 1
num_tags 16
title International Space Station conditions alter genomics proteomics and metabolomics in Aspergillus nidulans