LAMP assay for detection of Asian fish tapeworm

Data are relative fluorescent units obtained from the FAM channel of a AmpliFire Isothermal Fluorometer (Agdia, Inc., Elkhart, USA) recorded every 10 sec during as isothermal (LAMP) amplification. The isothermal reaction is to detect the Asian fish tapeworm, Schyzocotyle acheilognathi [syn. Bothriocephalus]. Samples (column headers) are described in tabs and include 1) positive control dilution series, 2) DNA Extractions from AFT sensitivity samples, and 3) DNA Extracted from non-AFT specificity controls.

Data and Resources

Field Value
accessLevel public
bureauCode {010:12}
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identifier USGS:5f862b1882cebef40f14c7a0
metadata_type geospatial
modified 20211012
old-spatial 0.0000, 0.0000, 0.0000, 0.0000
publisher U.S. Geological Survey
publisher_hierarchy Department of the Interior > U.S. Geological Survey
resource-type Dataset
source_datajson_identifier true
source_hash d62a33ecb784401ddb41d55465affa875576f63e
source_schema_version 1.1
spatial {"type": "Polygon", "coordinates": [[[0.0000, 0.0000], [0.0000, 0.0000], [ 0.0000, 0.0000], [ 0.0000, 0.0000], [0.0000, 0.0000]]]}
theme {geospatial}
Groups
  • AmeriGEOSS
  • National Provider
  • North America
Tags
  • amerigeo
  • amerigeoss
  • asian-fish-tapeworm
  • ckan
  • edna
  • environmental-dna
  • geo
  • geoss
  • invasive-species
  • isothermal-amplification
  • lamp-assay
  • loop-mediated-isothermal-amplification
  • molecular-detection
  • national
  • north-america
  • parasites
  • rapid-assay
  • united-states
  • usgs-5f862b1882cebef40f14c7a0
isopen False
license_id notspecified
license_title License not specified
maintainer Erik K Hofmeister
maintainer_email ehofmeister@usgs.gov
metadata_created 2025-11-22T03:30:48.958712
metadata_modified 2025-11-22T03:30:48.958716
notes Data are relative fluorescent units obtained from the FAM channel of a AmpliFire Isothermal Fluorometer (Agdia, Inc., Elkhart, USA) recorded every 10 sec during as isothermal (LAMP) amplification. The isothermal reaction is to detect the Asian fish tapeworm, Schyzocotyle acheilognathi [syn. Bothriocephalus]. Samples (column headers) are described in tabs and include 1) positive control dilution series, 2) DNA Extractions from AFT sensitivity samples, and 3) DNA Extracted from non-AFT specificity controls.
num_resources 2
num_tags 19
title LAMP assay for detection of Asian fish tapeworm