Methane Metabolism by Yeast

Our Technical Approach is to move the soluble Methane Monooxygenase system (sMMO) from methanotrophic bacteria into Pichi). sMMO consists of a hydroxylase (MMOH) assembled from 3 polypeptides, a reductase (MMOR), and a regulatory protein (MMOB). Thus 5 genes must be introduced into Pichia to enable oxidation of methane to methanol. Methanol then enters the natural metabolism of Pichia. Methods for introducing genes into Pichia are well developed, and we will apply these techniques to introduce the genes of the sMMO system into Pichia. We will judge the success of our efforts by growing Pichia containing sMMO with methane as a sole carbon source and comparing this to control strains. We will also measure incorporation of 13C methane into biomass.

Data and Resources

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identifier TECHPORT_93212
issued 2017-07-01
landingPage https://techport.nasa.gov/view/93212
modified 2020-01-29
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  • amerigeoss
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  • ckan
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  • geoss
  • national
  • north-america
  • united-states
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metadata_created 2025-11-23T00:17:10.815064
metadata_modified 2025-11-23T00:17:10.815068
notes Our Technical Approach is to move the soluble Methane Monooxygenase system (sMMO) from methanotrophic bacteria into Pichi). sMMO consists of a hydroxylase (MMOH) assembled from 3 polypeptides, a reductase (MMOR), and a regulatory protein (MMOB). Thus 5 genes must be introduced into Pichia to enable oxidation of methane to methanol. Methanol then enters the natural metabolism of Pichia. Methods for introducing genes into Pichia are well developed, and we will apply these techniques to introduce the genes of the sMMO system into Pichia. We will judge the success of our efforts by growing Pichia containing sMMO with methane as a sole carbon source and comparing this to control strains. We will also measure incorporation of 13C methane into biomass.
num_resources 4
num_tags 10
title Methane Metabolism by Yeast