Mucket eDNA detection in Wallen’s Bend, Clinch River, Tennessee, September 2019

The data describe the technical performance of a designed qPCR assay for the detection of mucket (Actinonaias ligamentina) eDNA from field collected water samples. Parameters described include the sequences of the primers and probes used; the limit of detection and limit of quantification for the assay; a list of freshwater mussels species that were used to test specificity of the assay; and in situ field samples that were tested to the verify assay.

Data and Resources

Field Value
accessLevel public
bureauCode {010:12}
catalog_@context https://project-open-data.cio.gov/v1.1/schema/catalog.jsonld
catalog_conformsTo https://project-open-data.cio.gov/v1.1/schema
catalog_describedBy https://project-open-data.cio.gov/v1.1/schema/catalog.json
identifier USGS:5f85c8b682cebef40f14c5d0
metadata_type geospatial
modified 20201119
old-spatial -83.0126, 36.579282, -83.004042, 36.583719
publisher U.S. Geological Survey
publisher_hierarchy Department of the Interior > U.S. Geological Survey
resource-type Dataset
source_datajson_identifier true
source_hash 4811a1a436fae10c82da419cca17a27348bca1c1
source_schema_version 1.1
spatial {"type": "Polygon", "coordinates": [[[-83.0126, 36.579282], [-83.0126, 36.583719], [ -83.004042, 36.583719], [ -83.004042, 36.579282], [-83.0126, 36.579282]]]}
theme {geospatial}
Groups
  • AmeriGEOSS
  • National Provider
  • North America
Tags
  • amerigeo
  • amerigeoss
  • assay-optimization
  • biota
  • cerc-molecular-lab
  • ckan
  • clinch-river
  • edna
  • freshwater-mussels
  • geo
  • geoss
  • national
  • north-america
  • qpcr
  • tennesee
  • united-states
  • usgs-5f85c8b682cebef40f14c5d0
  • wallens-bend
isopen False
license_id notspecified
license_title License not specified
maintainer Katy E Klymus
maintainer_email kklymus@usgs.gov
metadata_created 2025-11-20T04:17:17.152801
metadata_modified 2025-11-20T04:17:17.152805
notes The data describe the technical performance of a designed qPCR assay for the detection of mucket (Actinonaias ligamentina) eDNA from field collected water samples. Parameters described include the sequences of the primers and probes used; the limit of detection and limit of quantification for the assay; a list of freshwater mussels species that were used to test specificity of the assay; and in situ field samples that were tested to the verify assay.
num_resources 2
num_tags 18
title Mucket eDNA detection in Wallen’s Bend, Clinch River, Tennessee, September 2019