Multi-omic investigations of mouse liver subjected to simulated spaceflight freezing and storage protocols

This study compares standard laboratory protocols for tissue freezing and storage with a simulation of the delayed processing of liver specimens and long-term storage protocols used during the Rodent Research-1 (RR-1) payload. Liver samples from twelve-week old female C57BL/6J mice (snap frozen vs. 25 min. slow-frozen and stored for 3 days vs. 1 year) received from the RR-1 project were processed for extraction of RNA DNA and protein. RNA-seq whole genome bisulfite sequencing and mass spectrometry proteomics were performed. These multi-omics datasets will help distinguish the effects of freezing and storage time that could confound the interpretation of the Rodent Research-1 datasets.

Data and Resources

Field Value
accessLevel public
accrualPeriodicity irregular
bureauCode {026:00}
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identifier nasa_genelab_GLDS-49_em5f-7ccu
issued 2021-05-21
landingPage https://data.nasa.gov/dataset/multi-omic-investigations-of-mouse-liver-subjected-to-simulated-spaceflight-freezing-and-s
license http://www.usa.gov/publicdomain/label/1.0/
modified 2025-04-23
programCode {026:005}
publisher National Aeronautics and Space Administration
resource-type Dataset
source_datajson_identifier true
source_hash b1334d6d96f8c549a723c253ff3252dc031d60bed81ba5be608f6c7b3240df69
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theme {"Earth Science"}
Groups
  • AmeriGEOSS
  • National Provider
  • North America
Tags
  • AmeriGEO
  • AmeriGEOSS
  • CKAN
  • GEO
  • GEOSS
  • National
  • North America
  • United States
  • animal-and-tissue-sampling
  • data-transformation
  • extraction
  • freezing-profile
  • genelab-rnaseq-data-processing-protocol
  • labeling
  • library-construction
  • mass-spectrometry
  • nucleic-acid-extraction
  • nucleic-acid-sequencing
  • protein-extraction
  • sample-collection
  • sample-preservation-method
  • sample-storage-time
  • sequencing-library-construction
  • tmt-isobaric-labeling
isopen False
license_id us-pd
license_title us-pd
maintainer GeneLab Outreach
maintainer_email genelab-outreach@lists.nasa.gov
metadata_created 2025-09-24T18:55:26.836493
metadata_modified 2025-09-24T18:55:26.836501
notes This study compares standard laboratory protocols for tissue freezing and storage with a simulation of the delayed processing of liver specimens and long-term storage protocols used during the Rodent Research-1 (RR-1) payload. Liver samples from twelve-week old female C57BL/6J mice (snap frozen vs. 25 min. slow-frozen and stored for 3 days vs. 1 year) received from the RR-1 project were processed for extraction of RNA DNA and protein. RNA-seq whole genome bisulfite sequencing and mass spectrometry proteomics were performed. These multi-omics datasets will help distinguish the effects of freezing and storage time that could confound the interpretation of the Rodent Research-1 datasets.
num_resources 1
num_tags 24
title Multi-omic investigations of mouse liver subjected to simulated spaceflight freezing and storage protocols