Nitrogen and nitrogen isotope data for wheat and barley exposed to CeO2 nanoparticles

The effects of cerium oxide nanoparticles (CeO2-NPs) on 15N/14N ratio (δ15N) in wheat and barley were investigated. Seedlings were exposed to 0 and 500 mg CeO2-NPs/L (Ce-0 and Ce-500, respectively) in hydroponic suspension supplied with NH4NO3, NH4+, or NO3-. N uptake and δ15N discrimination (i.e. differences in δ15N of plant and δ15N of N source) were measured. Results showed that N content and 15N abundance decreased in wheat but increased in barley. Ce-500 only induced whole-plant δ15N discrimination (-1.48‰, P ≤ 0.10) with a simultaneous decrease (P ≤ 0.05) in whole-plant δ15N (-3.24‰) compared to Ce-0 (-2.74‰) in wheat in NH4+. Ce-500 decreased (P ≤ 0.01) root δ15N of wheat in NH4NO3 and NH4+ (3.23 and -2.25‰, respectively) compared to Ce-0 (4.96 and -1.27‰, respectively), but increased (P ≤ 0.05) root δ15N of wheat in NO3- (3.27‰) compared to Ce-0 (2.60‰). Synchrotron micro-XRF revealed the presence of CeO2-NPs in shoots of wheat and barley regardless of N source. Although the longer-term consequences of CeO2-NP exposure on N uptake and metabolism are unknown, the results clearly show the potential for ENMs to interfere with plant metabolism of critical plant nutrients such as N even when toxicity is not observed.

This dataset is associated with the following publication: Rico, C.M., M. Johnson, M.A. Marcus, and C.P. Andersen. Shifts in N and δ15N in wheat and barley exposed to cerium oxide nanoparticles. NanoImpact. Elsevier B.V., Amsterdam, NETHERLANDS, 11: 156-163, (2018).

Data and Resources

Field Value
accessLevel public
bureauCode {020:00}
catalog_conformsTo https://project-open-data.cio.gov/v1.1/schema
identifier https://doi.org/10.23719/1411219
license https://pasteur.epa.gov/license/sciencehub-license.html
modified 2018-03-15
programCode {020:095}
publisher U.S. EPA Office of Research and Development (ORD)
publisher_hierarchy U.S. Government > U.S. Environmental Protection Agency > U.S. EPA Office of Research and Development (ORD)
references {https://doi.org/10.1016/j.impact.2018.08.003}
resource-type Dataset
source_datajson_identifier true
source_hash c26d991080fc9e7910773fc5ceb5c0a85b2d4e58
source_schema_version 1.1
Groups
  • AmeriGEOSS
  • National Provider
  • North America
Tags
  • amerigeo
  • amerigeoss
  • ammonium-nitrate
  • ckan
  • engineered-nanomaterials-enms
  • geo
  • geoss
  • intergenerational-effects
  • isotope
  • isotopic-discrimination
  • national
  • nitrogen
  • north-america
  • synchrotron-micro-xrf
  • united-states
isopen False
license_id other-license-specified
license_title other-license-specified
maintainer Christian Andersen
maintainer_email andersen.christian@epa.gov
metadata_created 2025-11-21T14:05:46.071115
metadata_modified 2025-11-21T14:05:46.071119
notes The effects of cerium oxide nanoparticles (CeO2-NPs) on 15N/14N ratio (δ15N) in wheat and barley were investigated. Seedlings were exposed to 0 and 500 mg CeO2-NPs/L (Ce-0 and Ce-500, respectively) in hydroponic suspension supplied with NH4NO3, NH4+, or NO3-. N uptake and δ15N discrimination (i.e. differences in δ15N of plant and δ15N of N source) were measured. Results showed that N content and 15N abundance decreased in wheat but increased in barley. Ce-500 only induced whole-plant δ15N discrimination (-1.48‰, P ≤ 0.10) with a simultaneous decrease (P ≤ 0.05) in whole-plant δ15N (-3.24‰) compared to Ce-0 (-2.74‰) in wheat in NH4+. Ce-500 decreased (P ≤ 0.01) root δ15N of wheat in NH4NO3 and NH4+ (3.23 and -2.25‰, respectively) compared to Ce-0 (4.96 and -1.27‰, respectively), but increased (P ≤ 0.05) root δ15N of wheat in NO3- (3.27‰) compared to Ce-0 (2.60‰). Synchrotron micro-XRF revealed the presence of CeO2-NPs in shoots of wheat and barley regardless of N source. Although the longer-term consequences of CeO2-NP exposure on N uptake and metabolism are unknown, the results clearly show the potential for ENMs to interfere with plant metabolism of critical plant nutrients such as N even when toxicity is not observed. This dataset is associated with the following publication: Rico, C.M., M. Johnson, M.A. Marcus, and C.P. Andersen. Shifts in N and δ15N in wheat and barley exposed to cerium oxide nanoparticles. NanoImpact. Elsevier B.V., Amsterdam, NETHERLANDS, 11: 156-163, (2018).
num_resources 6
num_tags 15
title Nitrogen and nitrogen isotope data for wheat and barley exposed to CeO2 nanoparticles