Plasma Standards

Digital droplet PCR fluorsence amplitude values from which plasmid copy concentrations of DNA standards were determined as described in D-EMMD-MEB-018-SOP-01and Journal article. Summary of stability testing results also provided.

This dataset is associated with the following publication: Sivaganesan, M., M. Varma, S. Siefring, and R. Haugland. Quantification of plasmid DNA standards for U.S. EPA fecal indicator bacteria qPCR methods by droplet digital PCR analysis. JOURNAL OF MICROBIOLOGICAL METHODS. Elsevier Science Ltd, New York, NY, USA, 152: 135-142, (2018).

Data and Resources

Field Value
accessLevel public
bureauCode {020:00}
catalog_conformsTo https://project-open-data.cio.gov/v1.1/schema
identifier https://doi.org/10.23719/1500439
license https://pasteur.epa.gov/license/sciencehub-license.html
modified 2014-09-22
programCode {020:096}
publisher U.S. EPA Office of Research and Development (ORD)
publisher_hierarchy U.S. Government > U.S. Environmental Protection Agency > U.S. EPA Office of Research and Development (ORD)
references {https://doi.org/10.1016/j.mimet.2018.07.005}
resource-type Dataset
source_datajson_identifier true
source_hash b730b1ffb4614835c3a2ff8712d1e9937656a8e8
source_schema_version 1.1
Groups
  • AmeriGEOSS
  • National Provider
  • North America
Tags
  • AmeriGEO
  • AmeriGEOSS
  • CKAN
  • GEO
  • GEOSS
  • National
  • North America
  • United States
  • digital-droplet-pcr
  • dna-standards
  • draft-method-c
  • epa-methods-1609-1
  • epa-methods-1611-1
  • qpcr
isopen False
license_id other-license-specified
license_title other-license-specified
maintainer Richard Haugland
maintainer_email haugland.rich@epa.gov
metadata_created 2025-09-24T21:10:38.824744
metadata_modified 2025-09-24T21:10:38.824753
notes Digital droplet PCR fluorsence amplitude values from which plasmid copy concentrations of DNA standards were determined as described in D-EMMD-MEB-018-SOP-01and Journal article. Summary of stability testing results also provided. This dataset is associated with the following publication: Sivaganesan, M., M. Varma, S. Siefring, and R. Haugland. Quantification of plasmid DNA standards for U.S. EPA fecal indicator bacteria qPCR methods by droplet digital PCR analysis. JOURNAL OF MICROBIOLOGICAL METHODS. Elsevier Science Ltd, New York, NY, USA, 152: 135-142, (2018).
num_resources 8
num_tags 14
title Plasma Standards