Raw absorbance values for serially diluted whole blood preserved in different lysis buffers for Hawaiian Island forest bird avian malaria detection, 2005-2006

It is unclear whether DNA lysis buffers used for preservation of whole blood samples from Hawaiian forest birds cause denaturation and loss of antigen binding capability of antibody molecules. If their antigen binding capability is not affected, then the samples can be used in serological assays to provide an independent assessment of the accuracy of PCR (polymerase chain reaction) diagnostic tests for avian malaria. This data set provides raw absorbance measurements that were collected with a BioRad Model 3550 ELISA plate reader at a wavelength setting of 405 nm for different dilutions of blood from infected Hawaii Amakihi. The blood was preserved in different lysis buffers or placed in phosphate buffered saline as an untreated control.

Data and Resources

Field Value
accessLevel public
bureauCode {010:12}
catalog_@context https://project-open-data.cio.gov/v1.1/schema/catalog.jsonld
catalog_conformsTo https://project-open-data.cio.gov/v1.1/schema
catalog_describedBy https://project-open-data.cio.gov/v1.1/schema/catalog.json
identifier USGS:5e98e23682ce172707f6f983
metadata_type geospatial
modified 20200827
old-spatial -156.2640, 18.8023, -154.6820, 20.3395
publisher U.S. Geological Survey
publisher_hierarchy Department of the Interior > U.S. Geological Survey
resource-type Dataset
source_datajson_identifier true
source_hash 33fa7e1ba801dde41a12434f32ea3e0c991be070
source_schema_version 1.1
spatial {"type": "Polygon", "coordinates": [[[-156.2640, 18.8023], [-156.2640, 20.3395], [ -154.6820, 20.3395], [ -154.6820, 18.8023], [-156.2640, 18.8023]]]}
theme {geospatial}
Groups
  • AmeriGEOSS
  • National Provider
  • North America
Tags
  • amerigeo
  • amerigeoss
  • avian-malaria
  • biota
  • chlorodrepanis-virens
  • ckan
  • elisa
  • enzyme-linked-immunosorbant-assay
  • geo
  • geoss
  • hakalau-forest-national-wildlife-refuge
  • hawaii
  • hawaii-amakihi
  • hawaiian-islands
  • immunoblot
  • lysis-buffer
  • national
  • north-america
  • pcr
  • plasmodium-relictum
  • polymerase-chain-reaction
  • united-states
  • usgs-5e98e23682ce172707f6f983
  • western-blot
isopen False
license_id notspecified
license_title License not specified
maintainer Carter T Atkinson
maintainer_email catkinson@usgs.gov
metadata_created 2025-11-22T10:30:08.345686
metadata_modified 2025-11-22T10:30:08.345690
notes It is unclear whether DNA lysis buffers used for preservation of whole blood samples from Hawaiian forest birds cause denaturation and loss of antigen binding capability of antibody molecules. If their antigen binding capability is not affected, then the samples can be used in serological assays to provide an independent assessment of the accuracy of PCR (polymerase chain reaction) diagnostic tests for avian malaria. This data set provides raw absorbance measurements that were collected with a BioRad Model 3550 ELISA plate reader at a wavelength setting of 405 nm for different dilutions of blood from infected Hawaii Amakihi. The blood was preserved in different lysis buffers or placed in phosphate buffered saline as an untreated control.
num_resources 2
num_tags 24
title Raw absorbance values for serially diluted whole blood preserved in different lysis buffers for Hawaiian Island forest bird avian malaria detection, 2005-2006