Raw sequencing and amplicon sequence variant data from bacterial communities shed by Montastraea cavernosa coral fragments into filtered seawater mesocosms

The files provided in this U.S. Geological Survey (USGS) data release (Kellogg and others, 2021) include an amplicon sequence variant (ASV) table and the raw 16S rRNA gene amplicon files from six microbial communities (Mcav17, Mcav18, McH-101, McH-103, McD-57, and McD-58) derived from mesocosms consisting of filtered seawater in which either healthy or diseased (stony coral tissue loss disease) fragments of Montastraea cavernosa had been incubated, as well as sequence files of a mock community and extraction kit blank. Mesocosms were inoculated at the Smithsonian Marine Station located in Fort Pierce, Florida, during two separate trips: one in October 2019 and the other in November 2020. The coral fragments were collected between April 2018 and November 2020, from various locations throughout the Florida Keys. Mesocosms were set up by placing the coral fragments into filtered seawater for 4-5 days and then the fragments were removed so the water could be processed. The mock community was sequenced to assess any biases in the sequencing technology, while extraction blanks were sequenced to identify any contaminants in the DNA extraction kit.

Data and Resources

Field Value
accessLevel public
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identifier USGS:83f95ddf-34dc-4dd1-a5c1-3db973a46f7d
metadata_type geospatial
modified 20211122
old-spatial -80.3106, 27.45956, -80.3106, 27.45957
publisher U.S. Geological Survey
publisher_hierarchy Department of the Interior > U.S. Geological Survey
resource-type Dataset
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spatial {"type": "Polygon", "coordinates": [[[-80.3106, 27.45956], [-80.3106, 27.45957], [ -80.3106, 27.45957], [ -80.3106, 27.45956], [-80.3106, 27.45956]]]}
theme {geospatial}
Groups
  • AmeriGEOSS
  • National Provider
  • North America
Tags
  • amerigeo
  • amerigeoss
  • atlantic-ocean
  • bacteria
  • biota
  • ckan
  • coelenterates
  • florida-keys
  • geo
  • geoss
  • laboratory-methods
  • marine-biology
  • microbiology
  • national
  • north-america
  • oceans
  • united-states
  • usgs-83f95ddf-34dc-4dd1-a5c1-3db973a46f7d
isopen False
license_id notspecified
license_title License not specified
maintainer Christina A. Kellogg
maintainer_email ckellogg@usgs.gov
metadata_created 2025-11-22T20:04:12.922274
metadata_modified 2025-11-22T20:04:12.922278
notes The files provided in this U.S. Geological Survey (USGS) data release (Kellogg and others, 2021) include an amplicon sequence variant (ASV) table and the raw 16S rRNA gene amplicon files from six microbial communities (Mcav17, Mcav18, McH-101, McH-103, McD-57, and McD-58) derived from mesocosms consisting of filtered seawater in which either healthy or diseased (stony coral tissue loss disease) fragments of Montastraea cavernosa had been incubated, as well as sequence files of a mock community and extraction kit blank. Mesocosms were inoculated at the Smithsonian Marine Station located in Fort Pierce, Florida, during two separate trips: one in October 2019 and the other in November 2020. The coral fragments were collected between April 2018 and November 2020, from various locations throughout the Florida Keys. Mesocosms were set up by placing the coral fragments into filtered seawater for 4-5 days and then the fragments were removed so the water could be processed. The mock community was sequenced to assess any biases in the sequencing technology, while extraction blanks were sequenced to identify any contaminants in the DNA extraction kit.
num_resources 2
num_tags 18
title Raw sequencing and amplicon sequence variant data from bacterial communities shed by Montastraea cavernosa coral fragments into filtered seawater mesocosms