Simultaneous determination of a suite of endogenous steroids by LC-APPI-MS: Application to the identification of endocrine disruptors in aquatic toxicology

A liquid chromatogrpahy mass spectrometry (LC-MS) method was developed for the analysis of 13 endogenous steroids. The method was validated using both fish plasma and fish holding water. The method was applied to the assessment of endocrine disruption by analyzing plasma of fathead minnows exposed to fadrozole, and by analyzing holding water from Japanese medaka exposed to fadrozole.

This dataset is associated with the following publication: Blackwell, B., and G. Ankley. Simultaneous determination of a suite of endogenous steroids by LC-APPI-MS: Application to the identification of endocrine disruptors in aquatic toxicology. Journal of Chromatography B. Elsevier Science Ltd, New York, NY, USA, 1163: 122513, (2021).

Data and Resources

Field Value
accessLevel public
bureauCode {020:00}
catalog_conformsTo https://project-open-data.cio.gov/v1.1/schema
identifier https://doi.org/10.23719/1519296
license https://pasteur.epa.gov/license/sciencehub-license.html
modified 2020-09-01
programCode {020:000}
publisher U.S. EPA Office of Research and Development (ORD)
publisher_hierarchy U.S. Government > U.S. Environmental Protection Agency > U.S. EPA Office of Research and Development (ORD)
references {https://doi.org/10.1016/j.jchromb.2020.122513}
resource-type Dataset
source_datajson_identifier true
source_hash a5ae40f1798dc7a20797d9a1ea4816b720ec7d50
source_schema_version 1.1
Groups
  • AmeriGEOSS
  • National Provider
  • North America
Tags
  • amerigeo
  • amerigeoss
  • ckan
  • endocrine-disrupting-chemicals
  • geo
  • geoss
  • liquid-chromatography-mass-spectrometry
  • national
  • north-america
  • steroids
  • united-states
isopen False
license_id other-license-specified
license_title other-license-specified
maintainer Brett Blackwell
maintainer_email blackwell.brett@epa.gov
metadata_created 2025-11-22T07:43:17.317399
metadata_modified 2025-11-22T07:43:17.317403
notes A liquid chromatogrpahy mass spectrometry (LC-MS) method was developed for the analysis of 13 endogenous steroids. The method was validated using both fish plasma and fish holding water. The method was applied to the assessment of endocrine disruption by analyzing plasma of fathead minnows exposed to fadrozole, and by analyzing holding water from Japanese medaka exposed to fadrozole. This dataset is associated with the following publication: Blackwell, B., and G. Ankley. Simultaneous determination of a suite of endogenous steroids by LC-APPI-MS: Application to the identification of endocrine disruptors in aquatic toxicology. Journal of Chromatography B. Elsevier Science Ltd, New York, NY, USA, 1163: 122513, (2021).
num_resources 1
num_tags 11
title Simultaneous determination of a suite of endogenous steroids by LC-APPI-MS: Application to the identification of endocrine disruptors in aquatic toxicology