Xenopus metamorphosis after neonicotinoid exposure

Xenopus laevis tadpoles were held in 4 replicate vivaria for each of 4 treatments of neonicotinoid pesticides and one control treatment for at least one month (Control media, thiamethoxam high concentration of 100 ppm, thiamethoxam low concentrations of 20 ppm, clothianidin high concentrations of 100 ppm, and clothianidin low concentration of 20 ppm). Water was sampled for chemical testing on Day 33. Between Day 1 and 44, instantaneous measures of length were collected on a random selection of tadpoles from each vivarium. On Day 44, tadpole length (mm), weight, and developmental stage (Nieuwkoop-Faber staging) were measured for all tadpoles in all vivaria (three of the measured individuals from each replicate treatment group (total n=12 for each treatment) were vivisected and liver, brain, and somatic tissue frozen in RNA/DNA shield for qPCR analyses for another study). On Day 44, tadpoles (n=5) which had reached NF stage 57 were transferred to other vivaria and remained in their original treatment solution through Day 76; these animals were monitored until metamorphosis.

Data and Resources

Field Value
accessLevel public
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identifier USGS:5ac7c121e4b0e2c2dd110a09
metadata_type geospatial
modified 20210723
old-spatial -94.0400, 28.9300, -88.8000, 33.0200
publisher U.S. Geological Survey
publisher_hierarchy Department of the Interior > U.S. Geological Survey
resource-type Dataset
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spatial {"type": "Polygon", "coordinates": [[[-94.0400, 28.9300], [-94.0400, 33.0200], [ -88.8000, 33.0200], [ -88.8000, 28.9300], [-94.0400, 28.9300]]]}
theme {geospatial}
Groups
  • AmeriGEOSS
  • National Provider
  • North America
Tags
  • amerigeo
  • amerigeoss
  • amphibian
  • biota
  • ckan
  • clothianidin
  • geo
  • geoss
  • metamorphosis
  • national
  • neonicotinoid
  • north-america
  • organism-growth-and-development
  • thiamethoxam
  • united-states
  • usgs-5ac7c121e4b0e2c2dd110a09
isopen False
license_id notspecified
license_title License not specified
maintainer Jill A Jenkins
maintainer_email jenkinsj@usgs.gov
metadata_created 2025-11-19T17:24:43.149160
metadata_modified 2025-11-19T17:24:43.149167
notes Xenopus laevis tadpoles were held in 4 replicate vivaria for each of 4 treatments of neonicotinoid pesticides and one control treatment for at least one month (Control media, thiamethoxam high concentration of 100 ppm, thiamethoxam low concentrations of 20 ppm, clothianidin high concentrations of 100 ppm, and clothianidin low concentration of 20 ppm). Water was sampled for chemical testing on Day 33. Between Day 1 and 44, instantaneous measures of length were collected on a random selection of tadpoles from each vivarium. On Day 44, tadpole length (mm), weight, and developmental stage (Nieuwkoop-Faber staging) were measured for all tadpoles in all vivaria (three of the measured individuals from each replicate treatment group (total n=12 for each treatment) were vivisected and liver, brain, and somatic tissue frozen in RNA/DNA shield for qPCR analyses for another study). On Day 44, tadpoles (n=5) which had reached NF stage 57 were transferred to other vivaria and remained in their original treatment solution through Day 76; these animals were monitored until metamorphosis.
num_resources 2
num_tags 16
title Xenopus metamorphosis after neonicotinoid exposure