Ceratocystis DNA Detection in Frass During Caged Sampler Comparison

We designed two new samplers for monitoring airborne particulates, including fungal and fern spores and plant pollen, that rely on natural wind currents (Passive Environmental Sampler) or a battery operated fan (Active Environmental Sampler). Both samplers are modeled after commercial devices such as the Rotorod® and the Burkard® samplers, but are more economical and require less maintenance than commercial devices. We compared our two new samplers to Rotorod® samplers using Xyleborus spp. boring dust known to contain ROD causing pathogens. The comparison was done in a large outdoor field cage to determine relative effectiveness of the three samplers for capturing windblown boring dust. The dataset contains results of quantitative PCR (qPCR) tests to determine presence of Ceratocystis lukuohia, Ceratocystis huliohia, and Metrosideros polymorpha DNA in the samplers after release of boring dust over the course of twelve trials where position of samplers was rotated.

Data and Resources

Field Value
accessLevel public
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identifier USGS:5c1c1c85e4b0708288c7ac14
metadata_type geospatial
modified 20200827
old-spatial -155.096827, 19.695607, -155.091505, 19.700617
publisher U.S. Geological Survey
publisher_hierarchy Department of the Interior > U.S. Geological Survey
resource-type Dataset
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theme {geospatial}
Groups
  • AmeriGEOSS
  • National Provider
  • North America
Tags
  • amerigeo
  • amerigeoss
  • ceratocystis
  • ckan
  • environment
  • environmental-dna
  • environmental-sampler
  • geo
  • geoss
  • metrosideros-polymorpha
  • national
  • north-america
  • ohia-lehua
  • rapid-ohia-death
  • rod
  • spore-traps
  • united-states
  • usgs-5c1c1c85e4b0708288c7ac14
isopen False
license_id notspecified
license_title License not specified
maintainer Kylle Roy
maintainer_email kroy@usgs.gov
metadata_created 2025-11-20T13:41:05.947733
metadata_modified 2025-11-20T13:41:05.947737
notes We designed two new samplers for monitoring airborne particulates, including fungal and fern spores and plant pollen, that rely on natural wind currents (Passive Environmental Sampler) or a battery operated fan (Active Environmental Sampler). Both samplers are modeled after commercial devices such as the Rotorod® and the Burkard® samplers, but are more economical and require less maintenance than commercial devices. We compared our two new samplers to Rotorod® samplers using Xyleborus spp. boring dust known to contain ROD causing pathogens. The comparison was done in a large outdoor field cage to determine relative effectiveness of the three samplers for capturing windblown boring dust. The dataset contains results of quantitative PCR (qPCR) tests to determine presence of Ceratocystis lukuohia, Ceratocystis huliohia, and Metrosideros polymorpha DNA in the samplers after release of boring dust over the course of twelve trials where position of samplers was rotated.
num_resources 2
num_tags 18
title Ceratocystis DNA Detection in Frass During Caged Sampler Comparison