CottonGen Primer3

Primer3web version 4.0.0 allows users to pick PCR primers from a DNA sequence based on criteria including:

oligonucleotide melting temperature, size, GC content, and primer-dimer possibilities,

PCR product size,

positional constraints within the source (template) sequence, and

possibilities for ectopic priming (amplifying the wrong sequence)

many other constraints.

All of these criteria are user-specifiable as constraints, and some are specifiable as terms in an objective function that characterizes an optimal primer pair. Resources in this dataset:Resource Title: Website Pointer for CottonGen Primer3. File Name: Web Page, url: https://www.cottongen.org/tools/primer3

Data and Resources

Field Value
accessLevel public
bureauCode {005:18}
catalog_@context https://project-open-data.cio.gov/v1.1/schema/catalog.jsonld
catalog_conformsTo https://project-open-data.cio.gov/v1.1/schema
catalog_describedBy https://project-open-data.cio.gov/v1.1/schema/catalog.json
identifier 10113/AA23039
license https://www.usa.gov/publicdomain/label/1.0/
modified 2024-02-13
programCode {005:040}
publisher Agricultural Research Service
resource-type Dataset
source_datajson_identifier true
source_hash b0b328502806333a0a370853bc3f9b9b1c1d001af9af4e539dc2223eadfdd8e3
source_schema_version 1.1
Groups
  • AmeriGEOSS
  • National Provider
  • North America
Tags
  • AmeriGEO
  • AmeriGEOSS
  • CKAN
  • GEO
  • GEOSS
  • National
  • North America
  • United States
  • ars
  • cottongen
  • data-gov
  • np301
  • online-database
  • primer3
isopen False
license_id us-pd
license_title us-pd
maintainer Main, Dorrie
maintainer_email dorrie@wsu.edu
metadata_created 2025-09-24T03:58:28.793120
metadata_modified 2025-09-24T03:58:28.793131
notes <p>Primer3web version 4.0.0 allows users to pick PCR primers from a DNA sequence based on criteria including:</p> <ul> <li> <p>oligonucleotide melting temperature, size, GC content, and primer-dimer possibilities,</p> </li> <li> <p>PCR product size,</p> </li> <li> <p>positional constraints within the source (template) sequence, and</p> </li> <li> <p>possibilities for ectopic priming (amplifying the wrong sequence)</p> </li> <li> <p>many other constraints.</p> </li> </ul> <p>All of these criteria are user-specifiable as constraints, and some are specifiable as terms in an objective function that characterizes an optimal primer pair.</p> <div><br>Resources in this dataset:</div><br><ul><li><p>Resource Title: Website Pointer for CottonGen Primer3.</p> <p>File Name: Web Page, url: <a href="https://www.cottongen.org/tools/primer3" target="_blank">https://www.cottongen.org/tools/primer3</a> </p></li></ul>
num_resources 1
num_tags 14
title CottonGen Primer3