formalin effects data

RNA sequencing data from paired: (1) fresh-frozen, (2) direct-fixed in formalin for 18 hours, (3) frozen then formalin-fixed, or (4) frozen then ethanol-fixed and paraffin-embedded (n=6/group/condition) tissue samples from the livers of mice exposed to 600 ppm phenobarbital vs. vehicle control for 7 days in drinking water. RNA sequencing data from paired fresh frozen and 18 hr. formalin-fixed paraffin-embedded liver tissue from mice administered 1500, 3000, and 6000 ppm DEHP or vehicle control for 7 days by dietary ingestion; or RNA sequencing data from paired fresh frozen, 18 hr. formalin fixed paraffin embedded, or 3 wk. formalin fixed paraffin embedded liver tissue from mice administered 8 mg/kg/d Furan or vehicle control for 3 weeks by gavage.

This dataset is associated with the following publication: Wehmas, L., S. Hester, and C.E. Wood. Direct Formalin Fixation Induces Widespread Transcriptomic Effects in Archival Tissue Samples. Scientific Reports. Nature Publishing Group, London, UK, 10: 14497, (2020).

Data and Resources

Field Value
accessLevel public
bureauCode {020:00}
catalog_conformsTo https://project-open-data.cio.gov/v1.1/schema
identifier https://doi.org/10.23719/1518503
license https://pasteur.epa.gov/license/sciencehub-license.html
modified 2020-04-21
programCode {020:095}
publisher U.S. EPA Office of Research and Development (ORD)
publisher_hierarchy U.S. Government > U.S. Environmental Protection Agency > U.S. EPA Office of Research and Development (ORD)
references {https://doi.org/10.1038/s41598-020-71521-w}
resource-type Dataset
source_datajson_identifier true
source_hash 501347efac97a6af8f5ab2aa3170545ca4198d70
source_schema_version 1.1
Groups
  • AmeriGEOSS
  • National Provider
  • North America
Tags
  • AmeriGEO
  • AmeriGEOSS
  • CKAN
  • GEO
  • GEOSS
  • National
  • North America
  • United States
  • dehp
  • formalin-fixed-paraffin-embedded-samples
  • furan
  • gene-expression-profiles
  • mode-of-action-moa
  • molecular-pathway-analysis
  • phenobarbital
  • rna-sequencing
isopen False
license_id other-license-specified
license_title other-license-specified
maintainer Leah Wehmas
maintainer_email wehmas.leah@epa.gov
metadata_created 2025-09-24T19:42:31.391887
metadata_modified 2025-09-24T19:42:31.391897
notes RNA sequencing data from paired: (1) fresh-frozen, (2) direct-fixed in formalin for 18 hours, (3) frozen then formalin-fixed, or (4) frozen then ethanol-fixed and paraffin-embedded (n=6/group/condition) tissue samples from the livers of mice exposed to 600 ppm phenobarbital vs. vehicle control for 7 days in drinking water. RNA sequencing data from paired fresh frozen and 18 hr. formalin-fixed paraffin-embedded liver tissue from mice administered 1500, 3000, and 6000 ppm DEHP or vehicle control for 7 days by dietary ingestion; or RNA sequencing data from paired fresh frozen, 18 hr. formalin fixed paraffin embedded, or 3 wk. formalin fixed paraffin embedded liver tissue from mice administered 8 mg/kg/d Furan or vehicle control for 3 weeks by gavage. This dataset is associated with the following publication: Wehmas, L., S. Hester, and C.E. Wood. Direct Formalin Fixation Induces Widespread Transcriptomic Effects in Archival Tissue Samples. Scientific Reports. Nature Publishing Group, London, UK, 10: 14497, (2020).
num_resources 5
num_tags 16
title formalin effects data