Fungicides in nectar and pollen collected by bumble bees in a cherry orchard

Lab-reared colonies of Bombus huntii (Hunt bumble bee) were deployed in a commercial cherry orchard in the spring of 2016. A fungicide formulation containing boscalid (25.2%) and pyraclostrobin (12.8%) was applied one time at the recommended label rate. Nectar and pollen were collected daily, beginning two days before spray application and continuing for ten days following. Nectar samples were extracted with acetonitrile and cleaned up using dispersive solid-phase extraction with primary-secondary amine, C18, and magnesium sulfate. Pollen samples were extracted with ethyl acetate. Both types of samples were analyzed by GC-MS/MS for boscalid and pyraclostrobin. Fungicide concentrations in nectar varied spatially (by hive) and temporally. Individual pollen balls were visually identified as to primary and secondary plant sources. Pollen primarily from cherries contained the highest concentrations of the fungicides. There are 2 csv sets related to this data release.

Data and Resources

Field Value
accessLevel public
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datagov_dedupe_retained 20220721212438
identifier USGS:5c7073c1e4b0fe48cb43fc52
metadata_type geospatial
modified 20200812
old-spatial {"type": "Polygon", "coordinates": [[[-122.67883300634, 42.089745379349], [-122.67883300634, 46.217549030497], [ -116.1309814441, 46.217549030497], [ -116.1309814441, 42.089745379349], [-122.67883300634, 42.089745379349]]]}
publisher U.S. Geological Survey
publisher_hierarchy Department of the Interior > U.S. Geological Survey
resource-type Dataset
source_datajson_identifier true
source_hash 0e5f62f06a54ed4fd9a2b377d604b01401fae9c4
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theme {geospatial}
Groups
  • AmeriGEOSS
  • National Provider
  • North America
Tags
  • agriculture
  • agrochemicals
  • amerigeo
  • amerigeoss
  • bombus
  • ckan
  • ecotoxicology
  • fungicides
  • geo
  • geoss
  • national
  • north-america
  • oregon
  • pesticide-and-herbicide-contamination
  • pesticides
  • spring
  • united-states
  • usgs-5c7073c1e4b0fe48cb43fc52
  • year
isopen False
license_id notspecified
license_title License not specified
maintainer Michelle L. Hladik
maintainer_email mhladik@usgs.gov
metadata_created 2025-11-22T05:48:56.148898
metadata_modified 2025-11-22T05:48:56.148902
notes Lab-reared colonies of Bombus huntii (Hunt bumble bee) were deployed in a commercial cherry orchard in the spring of 2016. A fungicide formulation containing boscalid (25.2%) and pyraclostrobin (12.8%) was applied one time at the recommended label rate. Nectar and pollen were collected daily, beginning two days before spray application and continuing for ten days following. Nectar samples were extracted with acetonitrile and cleaned up using dispersive solid-phase extraction with primary-secondary amine, C18, and magnesium sulfate. Pollen samples were extracted with ethyl acetate. Both types of samples were analyzed by GC-MS/MS for boscalid and pyraclostrobin. Fungicide concentrations in nectar varied spatially (by hive) and temporally. Individual pollen balls were visually identified as to primary and secondary plant sources. Pollen primarily from cherries contained the highest concentrations of the fungicides. There are 2 csv sets related to this data release.
num_resources 2
num_tags 19
title Fungicides in nectar and pollen collected by bumble bees in a cherry orchard