Reporting the limits of detection (LOD) and quantification (LOQ) for environmental DNA assays: Data

This data set was collected to provide examples and aid in developing a standardized way of determining LOD and LOQ for eDNA assays and has 3 data files. GEDWG_LOD_DATA3.csv is raw qPCR data from multiple labs running multiple standards of known concentration for eDNA assays they regularly use. Comparison-Data.csv is the merged data output from running a generic LOD/LOQ calculator script multiple times with different LOD model settings. The generic LOD/LOQ calculator script is available at: https://github.com/cmerkes/qPCR_LOD_Calc, and details about the multiple settings used are commented in the analysis script available at: https://github.com/cmerkes/LOD_Analysis

Data and Resources

Field Value
accessLevel public
bureauCode {010:12}
catalog_@context https://project-open-data.cio.gov/v1.1/schema/catalog.jsonld
catalog_conformsTo https://project-open-data.cio.gov/v1.1/schema
catalog_describedBy https://project-open-data.cio.gov/v1.1/schema/catalog.json
identifier USGS:5d36fbfce4b01d82ce8a6cbf
metadata_type geospatial
modified 20220428
old-spatial -180.0000, -90.0000, 180.0000, 90.0000
publisher U.S. Geological Survey
publisher_hierarchy Department of the Interior > U.S. Geological Survey
resource-type Dataset
source_datajson_identifier true
source_hash df72f8162776585b5336bba153e2790b85b8c12a
source_schema_version 1.1
spatial {"type": "Polygon", "coordinates": [[[-180.0000, -90.0000], [-180.0000, 90.0000], [ 180.0000, 90.0000], [ 180.0000, -90.0000], [-180.0000, -90.0000]]]}
theme {geospatial}
Groups
  • AmeriGEOSS
  • National Provider
  • North America
Tags
  • amerigeo
  • amerigeoss
  • biota
  • ckan
  • columbia-environmental-research-center-4200-e-new-haven-road-columbia-missouri-65201
  • department-of-biochemistry-and-microbiology-university-of-victoria-3800-finnerty-road-victoria-
  • detection
  • edna
  • engineers-research-and-development-center-3909-halls-ferry-road-vicksburg-mississippi-39180
  • environmental-dna
  • geo
  • geoss
  • invasive-species
  • national
  • national-wildlife-research-center-4101-laporte-avenue-fort-collins-colorado-80521
  • north-america
  • ontario-ministry-of-natural-resources-and-forestry-2140-east-bank-drive-peterborough-ontario-k9
  • pcr
  • school-of-the-environment-washington-state-university-100-dairy-road-pullman-washington-99164
  • united-states
  • upper-midwest-environmental-sciences-center-2630-fanta-reed-road-la-crosse-wisconsin-54603
  • usgs-5d36fbfce4b01d82ce8a6cbf
  • whitney-genetics-laboratory-555-lester-avenue-onalaska-wisconsin-54650
isopen False
license_id notspecified
license_title License not specified
maintainer Christopher M Merkes
maintainer_email cmerkes@usgs.gov
metadata_created 2025-11-22T13:37:10.852487
metadata_modified 2025-11-22T13:37:10.852491
notes This data set was collected to provide examples and aid in developing a standardized way of determining LOD and LOQ for eDNA assays and has 3 data files. GEDWG_LOD_DATA3.csv is raw qPCR data from multiple labs running multiple standards of known concentration for eDNA assays they regularly use. Comparison-Data.csv is the merged data output from running a generic LOD/LOQ calculator script multiple times with different LOD model settings. The generic LOD/LOQ calculator script is available at: https://github.com/cmerkes/qPCR_LOD_Calc, and details about the multiple settings used are commented in the analysis script available at: https://github.com/cmerkes/LOD_Analysis
num_resources 2
num_tags 23
title Reporting the limits of detection (LOD) and quantification (LOQ) for environmental DNA assays: Data