RR-1 and RR-3 mouse liver transcriptomics with and without ERCC control RNA spike-ins

Proper interpretation of RNA sequencing data requires an understanding of assay sensitivity and sources of variability. To this end the External RNA Control Consortium (ERCC) developed a standard set of 92 poly-adenylated RNA transcripts that are orthogonal to mammalian RNA that can be added to RNA extracts before library generation and sequencing. The presence of these RNA standards at known ratios improves interpretation of RNA sequencing datasets. To test the utility of the ERCC RNA controls total RNA extracted from mouse livers from the Rodent Research 1 (flight and ground groups) and Rodent Research 3 (flight and ground groups) missions was sequenced with and without the ERCC control RNA. To allow comparison within and between groups ERCC Mix 1 or Mix 2 were added to half of the samples from each group respectively.

Data and Resources

Field Value
accessLevel public
accrualPeriodicity irregular
bureauCode {026:00}
catalog_@context https://project-open-data.cio.gov/v1.1/schema/catalog.jsonld
catalog_@id https://data.nasa.gov/data.json
catalog_conformsTo https://project-open-data.cio.gov/v1.1/schema
catalog_describedBy https://project-open-data.cio.gov/v1.1/schema/catalog.json
identifier nasa_genelab_GLDS-168
issued 2018-06-26
landingPage https://data.nasa.gov/d/c3t7-3uv9
modified 2020-01-29
programCode {026:005}
publisher National Aeronautics and Space Administration
resource-type Dataset
source_datajson_identifier true
source_hash 5b0aba44f77c874f18db5e78e9d3c936c43304da
source_schema_version 1.1
theme {"Earth Science"}
Groups
  • AmeriGEOSS
  • National Provider
  • North America
Tags
  • amerigeo
  • amerigeoss
  • ckan
  • geo
  • geoss
  • library-construction
  • microgravity
  • national
  • north-america
  • nucleic-acid-extraction
  • nucleic-acid-sequencing
  • rna-library-construction
  • sample-collection
  • spike-in-control
  • spike-in-control-protocol
  • treatment
  • united-states
isopen False
license_id notspecified
license_title License not specified
maintainer GeneLab Outreach
maintainer_email genelab-outreach@lists.nasa.gov
metadata_created 2025-11-19T18:14:27.333459
metadata_modified 2025-11-19T18:14:27.333465
notes Proper interpretation of RNA sequencing data requires an understanding of assay sensitivity and sources of variability. To this end the External RNA Control Consortium (ERCC) developed a standard set of 92 poly-adenylated RNA transcripts that are orthogonal to mammalian RNA that can be added to RNA extracts before library generation and sequencing. The presence of these RNA standards at known ratios improves interpretation of RNA sequencing datasets. To test the utility of the ERCC RNA controls total RNA extracted from mouse livers from the Rodent Research 1 (flight and ground groups) and Rodent Research 3 (flight and ground groups) missions was sequenced with and without the ERCC control RNA. To allow comparison within and between groups ERCC Mix 1 or Mix 2 were added to half of the samples from each group respectively.
num_resources 1
num_tags 17
title RR-1 and RR-3 mouse liver transcriptomics with and without ERCC control RNA spike-ins