Rare Earth Desorption Test with Citrate and Bicarbonate

LBT (lanthanide binding tag) cells were grown overnight in LB media with 0.05% Amp. 1:100 subculture taken from overnights, grown for 2 hours. LBT was induced with 0.002% arabinose added for 3 hours. REE adsorption was done by combining 350 ul (0.25% 1M MES, 12.5 uM Tb, and 12.5 uM La or Cu in sterile DI water) and 350 ul (LBT cells with OD = 1 in 10 mM MES), reacted for approx. 30 min.

Following adsorption, citrate and bicarbonate solutions were used in desorption to recover rare earth from cell surface, and to further separate REE from non-REEs. The samples were then centrifuged and a fraction of the supernatant was collected for ICP-MS analysis.

Data e Risorse

Campo Valore
DOI 10.15121/1452718
accessLevel public
bureauCode {019:20}
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identifier https://data.openei.org/submissions/3641
issued 2017-06-01T06:00:00Z
landingPage https://gdr.openei.org/submissions/965
license https://creativecommons.org/licenses/by/4.0/
modified 2018-06-14T16:28:49Z
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programCode {019:006}
projectLead Holly Thomas
projectNumber LLNL FY17 AOP 2.5.1.12
projectTitle Extraction of Rare Earth Metals from Geothermal Fluids using Bioengineered Microbes
publisher Lawrence Livermore National Laboratory
resource-type Dataset
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Gruppi
  • AmeriGEOSS
  • National Provider
  • North America
Tag
  • adsorption
  • amerigeo
  • amerigeoss
  • bicarbonate
  • brine
  • centrifuge
  • citrate
  • ckan
  • desorption
  • energy
  • geo
  • geoss
  • geothermal
  • lanthanide-binding-tag
  • lbt
  • national
  • north-america
  • rare-earth
  • ree
  • resource-recovery
  • united-states
isopen True
license_id cc-by
license_title Creative Commons Attribution
license_url http://www.opendefinition.org/licenses/cc-by
maintainer Yongqin Jiao
maintainer_email jiao1@llnl.gov
metadata_created 2025-11-20T06:44:17.581048
metadata_modified 2025-11-20T06:44:17.581053
notes LBT (lanthanide binding tag) cells were grown overnight in LB media with 0.05% Amp. 1:100 subculture taken from overnights, grown for 2 hours. LBT was induced with 0.002% arabinose added for 3 hours. REE adsorption was done by combining 350 ul (0.25% 1M MES, 12.5 uM Tb, and 12.5 uM La or Cu in sterile DI water) and 350 ul (LBT cells with OD = 1 in 10 mM MES), reacted for approx. 30 min. Following adsorption, citrate and bicarbonate solutions were used in desorption to recover rare earth from cell surface, and to further separate REE from non-REEs. The samples were then centrifuged and a fraction of the supernatant was collected for ICP-MS analysis.
num_resources 1
num_tags 21
title Rare Earth Desorption Test with Citrate and Bicarbonate