Raw absorbance values from serially diluted uninfected whole blood in different lysis buffers for Hawaiian Island forest bird avian malaria detection, 2005-2006

It is unclear whether DNA lysis buffers used for preservation of whole blood samples from Hawaiian forest birds cause denaturation and loss of antigen binding capability of antibody molecules. If their antigen binding capability is not affected, then the samples can be used in serological assays to provide an independent assessment of the accuracy of PCR (polymerase chain reaction) diagnostic tests for avian malaria. This data set provides raw absorbance measurements that were collected with a BioRad Model 3550 ELISA plate reader at a wavelength setting of 405 nm for different dilutions of blood from an uninfected Hawaii Amakihi. The blood was preserved in different lysis buffers or placed in phosphate buffered saline as an untreated control.

Data e Risorse

Campo Valore
accessLevel public
bureauCode {010:12}
catalog_@context https://project-open-data.cio.gov/v1.1/schema/catalog.jsonld
catalog_conformsTo https://project-open-data.cio.gov/v1.1/schema
catalog_describedBy https://project-open-data.cio.gov/v1.1/schema/catalog.json
identifier USGS:5e98e24582ce172707f6f985
metadata_type geospatial
modified 20200827
old-spatial -156.2640, 18.8023, -154.6820, 20.3395
publisher U.S. Geological Survey
publisher_hierarchy Department of the Interior > U.S. Geological Survey
resource-type Dataset
source_datajson_identifier true
source_hash 4f7ccc71b723e4a2be0a465a5482f53c8871510b
source_schema_version 1.1
spatial {"type": "Polygon", "coordinates": [[[-156.2640, 18.8023], [-156.2640, 20.3395], [ -154.6820, 20.3395], [ -154.6820, 18.8023], [-156.2640, 18.8023]]]}
theme {geospatial}
Gruppi
  • AmeriGEOSS
  • National Provider
  • North America
Tag
  • amerigeo
  • amerigeoss
  • avian-malaria
  • biota
  • chlorodrepanis-virens
  • ckan
  • elisa
  • enzyme-linked-immunosorbant-assay
  • geo
  • geoss
  • hakalau-forest-national-wildlife-refuge
  • hawaii
  • hawaii-amakihi
  • hawaiian-islands
  • immunoblot
  • lysis-buffer
  • national
  • north-america
  • pcr
  • plasmodium-relictum
  • polymerase-chain-reaction
  • united-states
  • usgs-5e98e24582ce172707f6f985
  • western-blot
isopen False
license_id notspecified
license_title License not specified
maintainer Carter T Atkinson
maintainer_email catkinson@usgs.gov
metadata_created 2025-11-22T02:04:03.968195
metadata_modified 2025-11-22T02:04:03.968199
notes It is unclear whether DNA lysis buffers used for preservation of whole blood samples from Hawaiian forest birds cause denaturation and loss of antigen binding capability of antibody molecules. If their antigen binding capability is not affected, then the samples can be used in serological assays to provide an independent assessment of the accuracy of PCR (polymerase chain reaction) diagnostic tests for avian malaria. This data set provides raw absorbance measurements that were collected with a BioRad Model 3550 ELISA plate reader at a wavelength setting of 405 nm for different dilutions of blood from an uninfected Hawaii Amakihi. The blood was preserved in different lysis buffers or placed in phosphate buffered saline as an untreated control.
num_resources 2
num_tags 24
title Raw absorbance values from serially diluted uninfected whole blood in different lysis buffers for Hawaiian Island forest bird avian malaria detection, 2005-2006