Effect of simulated microgravity on E. coli K12 MG1655 growth and gene expression

This study demonstrates simulated microgravity effects on E. coli K 12 MG1655 when grown on LB medium supplemented with glycerol. The results imply that E. coli readily reprograms itself to combat the multiple stresses imposed due to microgravity. Under these conditions it survives by upregulating oxidative stress protecting genes and simultaneously down regulating the membrane transporters and synthases to maintain cell homeostasis. In this study a clinostat that mimics microgravity conditions was used to investigate the effects of microgravity on E. coli grown in LB medium supplemented with glycerol to monitor the effects on growth and global gene expression using Affymetrix DNA microarrays.

Data e Risorse

Campo Valore
accessLevel public
accrualPeriodicity irregular
bureauCode {026:00}
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identifier nasa_genelab_GLDS-43_3hyu-yecm
issued 2021-05-21
landingPage https://data.nasa.gov/dataset/effect-of-simulated-microgravity-on-e-coli-k12-mg1655-growth-and-gene-expression
license http://www.usa.gov/publicdomain/label/1.0/
modified 2025-04-23
programCode {026:005}
publisher National Aeronautics and Space Administration
resource-type Dataset
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theme {"Earth Science"}
Gruppi
  • AmeriGEOSS
  • National Provider
  • North America
Tag
  • AmeriGEO
  • AmeriGEOSS
  • CKAN
  • GEO
  • GEOSS
  • National
  • North America
  • United States
  • data-transformation
  • feature_extraction
  • genelab-microarray-data-processing-protocol
  • grow
  • hybridization
  • image_acquisition
  • labeling
  • microgravity-simulation
  • nucleic_acid_extraction
  • p-gse40648-2
  • p-gse40648-3
  • p-gse40648-4
  • p-gse40648-5
  • p-gse40648-6
  • p-gse40648-7
  • p-gse40648-8
  • specified_biomaterial_action
isopen False
license_id us-pd
license_title us-pd
maintainer GeneLab Outreach
maintainer_email genelab-outreach@lists.nasa.gov
metadata_created 2025-09-24T04:56:14.902621
metadata_modified 2025-09-24T04:56:14.902630
notes This study demonstrates simulated microgravity effects on E. coli K 12 MG1655 when grown on LB medium supplemented with glycerol. The results imply that E. coli readily reprograms itself to combat the multiple stresses imposed due to microgravity. Under these conditions it survives by upregulating oxidative stress protecting genes and simultaneously down regulating the membrane transporters and synthases to maintain cell homeostasis. In this study a clinostat that mimics microgravity conditions was used to investigate the effects of microgravity on E. coli grown in LB medium supplemented with glycerol to monitor the effects on growth and global gene expression using Affymetrix DNA microarrays.
num_resources 1
num_tags 25
title Effect of simulated microgravity on E. coli K12 MG1655 growth and gene expression