HARV replication of the BRIC-21 Bacillus subtilis spaceflight samples

Previous spaceflight experiments using Bacillus subtilis have reported altered transcriptome profiles during spaceflight compared to matching ground samples. This study tried to replicate those transcriptome changes using High Aspect Ratio Vessels (HARVs). B. subtilis spores were grown in HARVs using the same protocol and growth conditions as the BRIC-21 spaceflight mission. RNA was extracted from the HARV samples and sequenced for differential expression analysis. HARV differentially expressed genes were then compared to the genes identified as differentially expressed in the BRIC-21 mission to access the accuracy of HARVs as a model for spaceflight experiments.

Data e Risorse

Campo Valore
accessLevel public
accrualPeriodicity irregular
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identifier nasa_genelab_GLDS-215_je2j-67g7
issued 2021-05-21
landingPage https://data.nasa.gov/dataset/harv-replication-of-the-bric-21-bacillus-subtilis-spaceflight-samples
license http://www.usa.gov/publicdomain/label/1.0/
modified 2025-04-23
programCode {026:005}
publisher National Aeronautics and Space Administration
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Gruppi
  • AmeriGEOSS
  • National Provider
  • North America
Tag
  • AmeriGEO
  • AmeriGEOSS
  • CKAN
  • GEO
  • GEOSS
  • National
  • North America
  • United States
  • library-construction
  • microgravity-simulation
  • nucleic-acid-extraction
  • nucleic-acid-sequencing
  • treatment-protocol
isopen False
license_id us-pd
license_title us-pd
maintainer GeneLab Outreach
maintainer_email genelab-outreach@lists.nasa.gov
metadata_created 2025-09-24T02:28:56.373742
metadata_modified 2025-09-24T02:28:56.373751
notes Previous spaceflight experiments using Bacillus subtilis have reported altered transcriptome profiles during spaceflight compared to matching ground samples. This study tried to replicate those transcriptome changes using High Aspect Ratio Vessels (HARVs). B. subtilis spores were grown in HARVs using the same protocol and growth conditions as the BRIC-21 spaceflight mission. RNA was extracted from the HARV samples and sequenced for differential expression analysis. HARV differentially expressed genes were then compared to the genes identified as differentially expressed in the BRIC-21 mission to access the accuracy of HARVs as a model for spaceflight experiments.
num_resources 1
num_tags 13
title HARV replication of the BRIC-21 Bacillus subtilis spaceflight samples